Sexual Plant Reproduction

, Volume 9, Issue 4, pp 189–196

Gene dispersal from transgenic crops

II. Hybridization between oilseed rape and the wild hoary mustard
Original Paper

DOI: 10.1007/BF02173097

Cite this article as:
Lefol, E., Fleury, A. & Darmency, H. Sexual Plant Reprod (1996) 9: 189. doi:10.1007/BF02173097

Abstract

The risk of release of genetically modified oilseed rape (Brassica napus) was investigated in relation to interspecific gene flow with hoary mustard (Hirschfeldia incana). Microscopic studies showed polymorphism within the population of hoary mustard for pollen germination on oilseed rape flowers. The transgenic herbicide-resistant and a commercial cultivar of oilseed rape were not different for pollen behaviour and ovule fertilization. Pollen tube growth was slow and erratic in interspecific crosses. Fertilization efficiency of oilseed rape and hoary mustard pollen in interspecific crosses was 15% and 1.3%, respectively, of that in intraspecific crosses. This unequal efficiency in reciprocal crosses was confirmed by hybrid seed set in pods. There was no post-zygotic barrier to the development of hybrid embryos in hoary mustard pods. Up to 26 spontaneous hybrids per male sterile oilseed rape plant, and one per hoary mustard plant, were obtained in field experiments. Hybrids were identified by isozyme electrophoresis, morphology and cytology. All hybrids were triploid with 26 chromosomes, and had low fertility. They produced 0.5 seeds per plant after spontaneous backcrossing with hoary mustard. Some of these descendants were produced from unreduced gametes. Our results suggest that gene flow is likely to occur, but its actual frequency under crop growing conditions remains to be estimated.

Key words

Biosafety Gene flow Hoary mustard Interspecific hybrid Oilseed rape Pollination 

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  1. 1.Laboratoire de MalherbologieInstitut National de la Recherche AgronomiqueDijonFrance

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