Young plants ofCoffea arabica were fed 200 µC C14O2 each, the carbon dioxide being absorbed by photosynthesis. The specific activity of the alkaloid trigonelline contained in the leaves was shown to be strongly dependent of leaf age. Excised plants without root system or plants with an interrupted shoot-root connection as regards pholem transport (by girdling the stem below the cotyledons) incorporate more radioactive carbon into the molecule of trigonelline than intact plants.
F. C. J. Zeijlemaker, Acta bot. neerl.2, 123 (1953).
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Blaim, K. Einfluss der Wurzeln auf die Trigonellinsynthese beiCoffea arabica . Experientia 16, 454 (1960). https://doi.org/10.1007/BF02171148