Advertisement

Experientia

, Volume 8, Issue 4, pp 143–145 | Cite as

On the desoxyribonucleic acid content of sea urchin gametes

  • D. Elson
  • E. Chargaff
Kurze Mitteilungen

Keywords

Acid Content Thymine Desoxyribonucleic Acid Desoxyribonucleic Acid Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

Nous avons déterminé les quantités d'acide désoxyribonucléique contenues dans les spermatozoïdes et les œufs vierges de l'ourisnParacentrotus lividus. Ces déterminations, faites au moyen d'une méthode microbiologique pour le dosage de la thymine, ont donné les résultats suivants: sperme 1,0 × 10−6, œuf environ 25 × 10−6 µg d'acide désoxyribonucléique par cellule.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 2.
    A. Boivin, R. Vendrely, andC. Vendrely, C. r. Acad. Sci. Paris226, 1061 (1948).Google Scholar
  2. 1.
    G. Schmidt andS. J. Thannhauser, J. Biol. Chem.161, 83 (1945).Google Scholar
  3. 2.
    G. Schmidt, L. Hecht, andS. J. Thannhauser, J. Gen. Physiol.31, 203 (1948).Google Scholar
  4. 3.
    R. K. Crane, Biol. Bull.93, 192 (1947).Google Scholar
  5. 3a.
    Calculations based onR. Ballentine, J. Cell. Comp. Physiol.15, 121 (1940).Google Scholar
  6. 4.
    Z. Dische, Mikrochemie8, 4 (1930).Google Scholar
  7. 5.
    C. Vendrely andR. Vendrely, C. r. Soc. Biol.143, 1386 (1949).Google Scholar
  8. 6.
    R. R. Roepke andF. E. Mercer, J. Bact.54, 731 (1947).Google Scholar
  9. 7.
    R. R. Roepke, R. L. Libby, andM. H. Small, J. Bact.48, 401 (1944).Google Scholar
  10. 8.
    We are greatly indebted to Prof.J. Runnström and Dr.T. Gustafson of the Wenner-Gren Institute of Experimental Biology, University of Stockholm, for these preparations.Google Scholar
  11. 9.
    G. Schmidt andS. J. Thannhauser, J. Biol. Chem.161, 83 (1945).Google Scholar
  12. 10.
    W. C. Schneider, J. Biol. Chem.161, 293 (1945).Google Scholar
  13. 11.
    E. Vischer andE. Chargaff, J. Biol. Chem.176, 715 (1948).Google Scholar
  14. 1.
    E. Vischer andE. Chargaff, J. Biol. Chem.176, 703 (1948).Google Scholar
  15. 2.
    E. Chargaff, R. Lipshitz, andC. Green, J. Biol. Chem.195, 155 (1952).Google Scholar
  16. 1.
    L. O. Öhman, Arkiv Zool. [A]36, No. 7 (1944).Google Scholar
  17. 2.
    E. Vischer andE. Chargaff, J. Biol. Chem.176, 703, 715 (1948).Google Scholar
  18. 2a.
    E. Chargaff, E. Vischer, R. Doniger, C. Green, andF. Misani, J. Biol. Chem.177, 405 (1949).Google Scholar
  19. 2b.
    E. Chargaff, R. Lipshitz, C. Green, andM. E. Hodes, J. Biol. Chem.192, 223 (1951).Google Scholar
  20. 2c.
    Compare alsoE. Chargaff, Exper.6, 201 (1950); J. Cell. and Comp. Physiol.38, suppl. 1, 41 (1951); Federation Proc.10, 654 (1951).Google Scholar
  21. 3.
    F. Schrader, Science114, 486 (1951).Google Scholar
  22. 3a.
    H. L. Fraenkel-Conrat, W. H. Ward, N. S. Snell, andE. D. Ducay, J. Am. Chem. Soc.72, 3826 (1950).Google Scholar
  23. 3b.
    H. L. Fraenkel-Conrat andE. D. Ducay, Biochem. J.49, XXXIX (1951).Google Scholar
  24. 4.
    Note added in proof.—A microbiological assay of desoxyribosides and its application to their estimation in frog's eggs have come to our attention after the submission of this paper:E. Hoff-Jørgensen, Biochem. J.50, 400 (1952).Google Scholar
  25. 4a.
    E. Hoff-Jørgensen andE. Zeuthen, Nature169, 245 (1952).Google Scholar

Copyright information

© Birkhäuser Verlag 1952

Authors and Affiliations

  • D. Elson
    • 1
  • E. Chargaff
    • 1
  1. 1.Cell Chemistry Laboratory, Department of Biochemistry, College of Physicians and SurgeonsColumbia UniversityNew York 32

Personalised recommendations