Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Die Wirkung energiereicher Strahlen auf die Atmungsfermentkette

  • 9 Accesses

  • 12 Citations

Summary

The irradiation sensitivity of the cytochrome system was investigated, using as examples baker's yeast andE. coli. A method is described with which the proportion of reduced cytochrome in cell suspensions could be measured spectrometrically. In contrast to isolated cytochrome, which is easily oxydized by Roentgen rays, the cytochrome of living cells is very stable to Roentgen rays. The electrone transport is not influenced by high energy rays. It is probable that the hydrogen transport is also undisturbed. Only if the cell is so damaged by high doses of rays that every exchange of substances is impossible, i.e. if the cell is dead, is the ferrocytochrome oxydized and perhaps also destroyed. The oxydation is thus a secondary process resulting from death of the cell by rays. It is supposed that the changes in energy metabolism described in the literature, especially the disturbances of oxydative phosphorylation, are also secondary processes.

The concepts developed in radiochemistry on the action of high energy rays on organic compounds must therefore be especially tested in each case for their application in the living cell.

This is a preview of subscription content, log in to check access.

Rerefences

  1. 1

    U. Hagen, Z. Naturforsch.10b, 210 (1955); Naturwissenschaften42, 185 (1955).

  2. 1a

    E. S. G. Barron undV. Flood, Arch. Biochem. Biophys.41, 203 (1952).

  3. 2

    E. S. G. Barron, B. Gasvoda undV. Flood, Biol. Bull.97, 51, 144 (1949).

  4. 2a

    E. S. G. Barron undL. Seki, J. gen. Physiol.35, 865 (1952).

  5. 3

    E. J. Boell, J. cell. comparat. Physiol. [39. Suppl.]2, 19 (1952).

  6. 3a

    D. Billen, G. E. Stapleton undA. Hollaender, J. Bacteriol.65, 131 (1953).

  7. 3b

    M. le May, Proc. Soc. exper. Biol. Med.77, 337 (1951).

  8. 3c

    H. Laser, Nature174, 753 (1954).

  9. 4

    U. Hagen, Z. Naturforsch.10b, 210 (1955); Naturwissenschaften42, 185 (1955).

  10. 2

    O. Warburg,Schwermetalle als Wirkungsgruppen von Fermenten (Editio Cantor, Aulendorf i. Wttbg. 1949).

  11. 3

    J. Lindenmann, Schweiz. Z. allg. Path. Bakt.17, 311 (1954).

  12. 4

    D. Keilin undE. F. Hartree, Proc. roy. Soc. London [B]127, 167 (1939).

  13. 1

    D. Keilin undC. H. Harpley, Biochem. J.35, 688 (1941).

  14. 1

    B. Flaschenträger undE. Lehnartz,Physiologische Chemie, Bd. 1 (Berlin 1951), S. 1186, Tab. 191.

  15. 1

    L. Martin undJ. Brachet, C. r. Soc. Biol.1946, 1201.

  16. 1a

    L. Martin undP. Gérard, C. r. Soc. Biol.1946, 1245.

  17. 2

    R. Glocker, H. Langendorff undA. Reuss, Strahlentherapie46, 517 (1932).

  18. 3

    H. Langendorff undM. Langendorff undK. Sommermeyer, Z. Naturforsch.8b, 117 (1953).

  19. 4

    A. Hollaender, Symp. Radiobiol. Liège 1954.

  20. 5

    E. J. Boell, J. cell. comp. Physiol. [39. Suppl.]2, 19 (1952).

  21. 5a

    D. Billen, G. E. Stapleton undA. Hollaender, J. Bacteriol.65, 131 (1953).

  22. 5b

    M. le May, Proc. Soc. exper. Biol. Med.77, 337 (1951).

  23. 5c

    H. Laser, Nature174, 753 (1954).

  24. 1

    J. Lindenmann, Schweiz. Z. allg. Path. Bakt.17, 311 (1954).

  25. 1

    E. S. G. Barron, B. Gasvoda undV. Flood, Biol. Bull.97, 51, 144 (1949).

  26. 1a

    E. S. G. Barron undL. Seki, J. gen. Physiol.35, 865 (1952).

  27. 2

    E. S. G. Barron undV. Flood, Arch. Biochem. Biophys.41, 203 (1952).

  28. 3

    U. Hagen, Z. Naturforsch.10b, 250 (1955); Naturwissenschaften42, 185 (1955).

  29. 4

    E. J. Boell, J. cell. comp. Physiol. [39. Suppl.]2, 19 (1952).

  30. 4a

    D. Billen, G. E. Stapleton undA. Hollaender, J. Bacteriol.65, 131 (1953).

  31. 4b

    M. Le May, Proc. Soc. exper. Biol. Med.77, 337 (1951).

  32. 4c

    H. Laser, Nature174, 753 (1954).

  33. 1

    E. J. Boell, J. cell. comp. Physiol., 39. Suppl.2, 19 (1952).

  34. 1a

    D. Billen, G. E. Stapleton undA. Hollaender, J. Bacteriol.65, 131 (1953).

  35. 1b

    M. le May, Proc. Soc. exper. Biol. Med.77, 337 (1951).

  36. 1c

    H. Laser, Nature174, 753 (1954).

  37. 2

    M. Errera, Symp. Radiobiol. Liège 1954.

  38. 3

    G. Ashwell undJ. Hickmann, Proc. Soc. exper. Biol. Med.80, 407 (1952).

  39. 3a

    E. Maxwell undG. Ashwell, Arch. Biochem. Biophys.43, 389 (1953).

  40. 3b

    R. L. Potter undF. H. Bethell, Federat. Proc.11, 270 (1952).

  41. 3c

    J. F. Thomson, W. W. Tourtellotte undM. G. Carttar, Proc. Soc. exper. Biol. Med.80, 268 (1952).

  42. 3d

    D. W. van Bekkum, H. J. Jongepier, H. T. M. Nieuwekerk undJ. A. Cohen, Brit. J. Radiol.27, 127 (1954).

  43. 3e

    D. W. van Bekkum, Symp. Radiobiol. Liège 1954; Biochim. biophys. Acta16, 437 (1955).

  44. 4

    W. Florsheim, C. Doernbach undM. E. Morton, Proc. Soc. exper. Biol. Med.81, 121 (1952).

  45. 5

    D. Billen, B. L. Strehler, G. E. Stapleton undE. Brigham Arch. Biochem. Biophys.43, 1 (1953).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Langendorff, H., Hagen, U. Die Wirkung energiereicher Strahlen auf die Atmungsfermentkette. Experientia 11, 485–489 (1955). https://doi.org/10.1007/BF02166833

Download citation