Journal of Applied Phycology

, Volume 4, Issue 3, pp 221–231 | Cite as

From open ponds to vertical alveolar panels: the Italian experience in the development of reactors for the mass cultivation of phototrophic microorganisms

  • M. R. Tredici
  • R. Materassi


The need to develop new concepts in reactor design and the growing interest inSpirulina prompted our group to abandon open ponds in the seventies and to focus interest mainly on closed systems. Two substantially different closed photobioreactors have been developed and are at present under investigation in our Research Centre: the tubular photobioreactor (made of rigid or collapsible tubes) and the recently devised vertical alveolar panel (VAP) made of 1.6-cm-thick Plexiglas alveolar sheets.

The technical characteristics of the two systems are described and discussed in relation to the main factors which regulate the growth of oxygenic photosynthetic microorganisms in closed reactors.

Key words

microalgal mass culture tubular photobioreactor vertical alveolar panel (VAP) 


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  1. Benemann JR (1989) The future of microalgal biotechnology. In Cresswell RC, Rees TAV, Shah N (eds), Algal and Cyanobacterial Biotechnology. Longman Scientific & Technical Press, Harlow, 317–337.Google Scholar
  2. Borowitzka LJ, Borowitzka MA (1989) Industrial production: methods and economics. In Cresswell RC, Rees TAV, Shah N (eds), Algal and Cyanobacterial Biotechnology. Longman Scientific & Technical Press, Harlow, 295–316.Google Scholar
  3. Chaumont D, Thepenier C, Gudin C, Junjas C (1988) Scaling up a tubular photoreactor for continuous culture ofPorphyridium cruentum. From laboratory to pilot plant (1981–1987) In Stadler T, Mollion J, Verdus MC, Karamanos Y, Morvan H, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science, London, 199–208.Google Scholar
  4. Cohen E, (Malis)Arad S (1989) A closed system for outdoor cultivation ofPorphyridium. Biomass 18: 59–67.Google Scholar
  5. Cook PM (1950) Large-scale culture ofChlorella. In Brunel J, Prescott GW, Tiffany LH (eds), The Culturing of Algae. The Charles F. Kettering Foundation, 53–75.Google Scholar
  6. Fisher AW (1956) Engineering for algae culture. In Proceedings of the World Symposium on Applied Solar Energy. Stanford Research Institute, Menlo Park (California), 243–253.Google Scholar
  7. Gudin C, Thepenier C, Chaumont D, Berson X (1988) Bioreactors. In Ramus J, Jones MC (eds), Polysaccharides from Microalgae: a New Agroindustry, Duke University Marine Laboratory, Beaufort, 133–141.Google Scholar
  8. Lee YK (1986) Enclosed bioreactors for the mass cultivation of photosynthetic microorganisms: the future trend. Trends in Biotechnol. 4(7): 186–189.Google Scholar
  9. Materassi R, Balloni W, Pushparaj B, Pelosi E, Sili C (1981) Coltura massiva diSpirulina in sistemi colturali aperti. In Materassi R (ed.), Atti del Convegno ‘Prospettive della Coltura di Spirulina in Italia’. CNR, Roma, 241–260.Google Scholar
  10. Materassi R, Tredici MR, Milicia F, Sili C, Pelosi E, Vincenzini M, Torzillo G, Balloni W, Florenzano G, Wagener K (1984) Development of a production size system for the mass culture of marine microalgae. In Palz W, Pirrwitz D (eds), Energy from Biomass, Series E, Vol. 5, D. Reidel Publishing Company, Dordrecht, 150–158.Google Scholar
  11. Miyamoto K, Wable O, Benemann JR (1988) Vertical tubular reactor for microalgae cultivation. Biotechnology Letters 10: 703–708.Google Scholar
  12. Pirt SJ, Lee YK, Walach MR, Pirt MW, Balyuzi HHM, Bazin MJ (1983) A tubular bioreactor for photosynthetic production of biomass from carbon dioxide: design and performance. J. Chem. Techn. Biotechnol. 33B: 35–58.Google Scholar
  13. Richmond A (1987) The challenge confronting industrial microagriculture: high photosynthetic efficiency in large-scale reactors. Hydrobiologia 151/152: 117–121.Google Scholar
  14. Richmond A (1990) Large scale microalgal culture and applications. In Round FE, Chapman DJ (eds), Progress in Phycological Research Vol. 7. Biopress Ltd, Bristol, 269–330.Google Scholar
  15. Richmond A, Lichtenberg E, Stahl B, Vonshak A (1990) Quantitative assessment of the major limitations on productivity ofSpirulina platensis in open raceways. J. appl. Phycol. 2: 195–206.Google Scholar
  16. Tomaselli L, Giovannetti L, Sacchi A, Bocci F (1988) Effects of temperature on growth and biochemical composition inSpirulina platensis strain M2. In Stadler T, Mollion J, Verdus M-C, Karamanos Y, Morvan H, Christiaen D (eds), Algal Biotechnology. Elsevier Applied Science, London, 305–314.Google Scholar
  17. Torzillo G, Pushparaj B, Bocci F, Balloni W, Materassi R, Florenzano G (1986) Production ofSpirulina biomass in closed photobioreactors. Biomass 11: 61–74.Google Scholar
  18. Tredici MR, Carlozzi P, Chini Zittelli G, Materassi R (1991) A vertical alveolar panel (VAP) for outdoor mass cultivation of microalgae and cyanobacteria. Bioresource Technology 38: 153–159.Google Scholar
  19. Weissman JC, Goebel RP, Benemann JR (1988) Photobioreactor design: mixing, carbon utilization, and oxygen accumulation. Biotech. Bioengng 31: 336–344.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • M. R. Tredici
    • 1
  • R. Materassi
    • 1
  1. 1.Centro di Studio dei Microrganismi Autotrofi (CNR) and Dipartimento di Scienze e Tecnologie Alimentari e MicrobiologicheUniversità di FirenzeFirenzeItaly

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