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Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol

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Studies of 24 strains ofBlastomyces dermatitidis confirmed previously published results that the yeast-phase of this fungus is more sensitive than the mycelial-phase to cycloheximide and chloramphenicol.

Studies of 5 strains each ofHistoplasma capsulatum, Paracoccidioides brasiliensis andSporotrichum schenckii show that that these species also have a similar yeast-phase mycelial -phase sensitivity differential in regard to these antibiotics.

A cycloheximide resistant strain ofB. dermatitidis was developed from a sensitive strain.

The experimental results support the general practice of using 0.5 mg/ml cycloheximide and 0.05 mg/ml chloramphenicol in media for the isolation of the four fungi at 25° C. The results indicate, however, that some strains would not be recovered at 37° C with similar concentrations of these antibiotics.

It is recommended that a concentration of not more than 0.2 mg/ml chloramphenicol should be used to preserve sputum which is subsequently to be cultured forB. dermatitidis, Histoplasma capsulatum, Paracoccidioides brasiliensis orS. schenckii.

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Mc Donough, E.S., Georg, L.K., Ajello, L. et al. Growth of dimorphic human pathogenicfungi on media containing cycloheximide and chloramphenicol. Mycopathologia et Mycologia Applicata 13, 113–120 (1960). https://doi.org/10.1007/BF02155508

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  • General Practice
  • Chloramphenicol
  • Resistant Strain
  • Cycloheximide
  • Similar Concentration