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Basal linear deposit in the human macula

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We used electron microscopy and immunohistochemistry to study the macular regions of nine enucleated elderly human eyes and to document the various abnormalities present in the so-called basal linear deposit. These changes include bush-like strands of electron-dense material, which project from the basement membrane of the retinal pigment epithelium, deposition of wide-banded collagen, vesiculoid elements, membrane-bound structures and occasional melanin granules. Fibronectin was also identified in the basal linear deposit and in Bruch's membrane, but mucopolysaccharides could not be demonstrated. The presence of electron-empty spaces suggests a disturbance in water permeability. Our studies also showed neovascularisation beneath the retinal pigment epithelium in locations where the basal linear deposit was abundant, as well as erosion of Bruch's membrane by macrophages and endothelial cell processes. Our findings suggest that the basal linear deposit is an important precursor of neovascularisation. Possible pathogenetic mechanisms are discussed.

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  1. Burns R, Feeney-Burns L (1980) Clinico-morphologic correlations of drusen of Bruch's membrane. Trans Am Ophthalmol Soc 78: 206–225

  2. Dušek J, Streicher T, Schmidt K (1982) Hereditäre Drusen der Bruchschen Membran. II. Untersuchung von Semidünnschnitten und elektronenmikroskopischen Ergebnissen. Klin Monatsbl Augenheilkd 181: 79–83

  3. Duvall J, Tso M (1985) Cellular mechanisms of resolution of drusen after laser coagulation: an experimental study. Arch Ophthalmol 103: 694–703

  4. Farkas T, Sylvester V, Archer D (1971a) The ultrastructure of drusen. Am J Ophthalmol 71: 1196–1205

  5. Farkas T, Sylvester V, Archer D, Altona M (1971b) The histochemistry of drusen. Am J Ophthalmol 71: 1206–1215

  6. Feeney-Burns L, Ellersieck M (1985) Age-related changes in the ultrastructure of Bruch's membrane. Am J Ophthalmol 100: 686–697

  7. Ferris F, Fine S, Hyman L (1984) Age-related macular degeneration and blindness due to neovascular maculopathy. Arch Ophthalmol 102: 1640–1642

  8. Foos R, Trese M (1982) Chorioretinal juncture. Vascularization of Bruch's membrane in peripheral fundus. Arch Ophthalmol 100: 1492–1503

  9. Frank R, Green WR, Pollack J (1973) Senile macular degeneration. Clinicopathological correlations of a case in the predisciform stage. Am J Ophthalmol 75: 587–594

  10. Friedman E, Smith TR, Kuwabara T (1963) Senile choroidal vascular patterns and drusen. Arch Ophthalmol 69: 114–124

  11. Garner A (1975) Pathology of macular degeneration in the elderly. Trans Ophthalmol Soc UK 95: 54–61

  12. Gass J (1967) Pathogenesis of disciform detachment of the neuro- epithelium. Am J Ophthalmol 63: 573–711

  13. Gass J (1973) Drusen and disciform macular detachment and degeneration. Arch Ophthalmol 90: 206–217

  14. Gass J, Jallow S, Davis B (1985) Adult vitelliform macular detachment occurring in patients with basal laminar drusen. Am J Ophthalmol 99: 445–459

  15. Green R, Key SN (1977) Senile macular degeneration: a histopathological study. Trans Am Ophthalmol Soc 75: 180–254

  16. Grindle CFJ, Marshall J (1978) Ageing changes in Bruch's membrane and their functional implications. Trans Ophthalmol Soc UK 98: 172–175

  17. Henkind P, Gartner S (1983) The relationship between retinal pigment epithelium and the choriocapillaris. Trans Ophthalmol Soc UK 103: 444–447

  18. Heriot W, Henkind P, Bellkorn R, Burns M (1984) Choroidal neovascularization can digest Bruch's membrane. A prior break is not essential. Ophthalmology 91: 1602–1608

  19. Hogan M (1967) Bruch's membrane and disease of the macula. Role of elastic tissue and collagen. Trans Ophthalmol Soc UK 87: 113–167

  20. Hogan M, Alvarado J (1967) Studies on the human macula. IV. Ageing changes in Bruch's membrane. Arch Ophthalmol 77: 410–420

  21. Kalebic T, Garbisa S, Glaser B, Liotta LA (1983) Basement membrane collagen: degradation by migrating endothelial cells. Science 221: 281–283

  22. Kenyon K, Maumenee AE, Ryan S, Whitmore P, Green WR (1985) Diffuse drusen and associated complications. Am J Ophthalmol 100: 119–128

  23. Killingsworth M, Sarks SH (1982) Giant cells in disciform macular degeneration of the human eye. Micron 13: 359–360

  24. Lerche W (1964) Elektronenmikroskopische Beobachtungen über altersbedingte Verä nderungen an der Bruchschen Membran des Menschen. Anat Gesell Verh 60: 123–132

  25. Lyda W, Eriksen N, Krishna N (1957) Studies of Bruch's membrane. Flow and permeability studies in a Bruch's membrane- choroid preparation. Am J Ophthalmol 44: 362–370

  26. Miller H, Miller B, Ryan S (1985) Correlation of choroidal subretinal neovascularization with fluorescein angiography. Am J Ophthalmol 99: 263–271

  27. Penfold P, Killingsworth M, Sarks SH (1984) An ultrastructural study of the role of leukocytes and fibroblasts in the breakdown of Bruch's membrane. Aust J Ophthalmol 12: 23–31

  28. Penfold P, Killingsworth M, Sarks SH (1985) Senile macular degeneration: the involvement of immunocompetent cells. Graefe's Arch Clin Exp Ophthalmol 223: 69–76

  29. Sarks SH (1973) New vessel formation beneath the retinal pigment epithelium in senile eyes. Br J Ophthalmol 57: 951–965

  30. Sarks SH (1976) Ageing and degeneration in the macular region: A clinico-pathological study. Br J Ophthalmol 60: 324–341

  31. Sarks SH (1980) Council Lecture. Drusen and their relationship to senile macular degeneration. Aust J Ophthalmol 8: 117–130

  32. Sarks SH, Van Driel D, Maxwell L, Killingsworth M (1980) Softening of drusen and subretinal neovascularisation. Trans Ophthalmol Soc UK 100: 414–422

  33. Small M, Green WR, Alpar J, Drewry R (1976) A clinicopathologic correlation of two cases with neovascularization beneath the retinal pigment epithelium. Arch Ophthalmol 94: 601–607

  34. Spitznas M (1974) The fine structure of the chorioretinal border tissues of the adult human eye. Adv Ophthalmol 28: 78–174

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Correspondence to K. U. Loffler.

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Loffler, K.U., Lee, W.R. Basal linear deposit in the human macula. Graefe's Arch Clin Exp Ophthalmol 224, 493–501 (1986). https://doi.org/10.1007/BF02154735

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  • Permeability
  • Endothelial Cell
  • Basement Membrane
  • Retinal Pigment Epithelium
  • Water Permeability