Histologic changes in the inner retina of albino rats following intravitreal injection of monosodiuml-glutamate
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Monosodiuml-glutamate is known to cause intracellular swelling, necrosis, and disappearance of most inner retinal neurons, with concomitant thinning of inner retinal layers within hours after subcutaneous injection into neonatal rodents. A similar process can be observed in adult rat retina after intravitreal glutamate injection. To better describe and compare this process with that reported after systemic application, adult Spraque-Dawley rat eyes were intravitreally injected with 1 μmol monosodiuml-glutamate and the retinas studied by LM and EM over a 2-month period. Results demonstrated that adult rat retina 6experienced severe degenerative changes which progressed in two stages: an initial stage of massive intracellular swelling and a second stage of necrosis and cell loss. Degeneration involved ganglion and inner nuclear layer cells. Inner retinal thickness decreased concurrently. By 2 months postinjection, degenerative changes in rod spherules and some loss of photoreceptor nuclei were observed. Both time course and severity of the lesion differed from that described in prior studies after systemic treatment.
KeywordsGlutamate Retina Subcutaneous Injection Degenerative Change Cell Loss
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- Cohen AI (1967) An electron microscopic study of the modification by monosodium glutamate of the retinas of normal and “rodless” mice. Am J Anat 120:319–356Google Scholar
- Lucas DR, Newhouse JP (1957) The toxic effect of sodium L-glutamate on the inner layers of the retina. Am Med Assoc Arch Ophthalmol 58:193–201Google Scholar
- Sisk DR, Kirsch AD, Kuwabara T (1984b) Regional differences in glutamate-induced retinal degeneration: a quantitative study in albino rats. Proc Int Soc Eye Res 3:39Google Scholar