Eight “chinchilla bastard” pigmented rabbits were treated intravenously with sonicated phospholipid liposomes (SUV) consisting predominantly of egg yolk phosphatidylcholine (PC). Four rabbits were treated with canthaxanthin incorporated into PC liposomes in a single injection and fractionated over a period of 19 days. The total amount of PC used was a single dose of 260 mg administered as a 50-ml suspension and 13.7 mg/fraction given as a 5-ml suspension. The total amount of canthaxanthin used was a single 40 mg as a 50-ml suspension and 2.1 mg/fraction as 5-ml suspension. Electroretinography showed that canthaxanthin produced a depression of thea-waves and prolongation of the scotopica- andb-wave peak latencies. A single high-dose injection of PC and PC plus canthaxanthin resulted in a transitory reduction in ERG amplitudes. A single injection of PC and canthaxanthin also produced hypernormala-waves within the recovery time. Electron microscopy demonstrated that in contrast to the controls (PC), the canthaxanthin-treated rabbits showed alterations in the RPE/photoreceptor complex, indicating that these layers are the primary site of canthaxanthin effects on the retina.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Carter-Dawson L, Kuwabara T, O'Brian PJ, Bieri JG (1979) Structural and biochemical changes in vitamin A-deficient rat retinas. Invest Ophthalmol Vis Sci 18: 437–446
Dowling JE, Wald G (1960) The biological function of vitamin A acid. Proc Natl Acad Sci USA 46: 587–608
Gee A, Deitz VR (1953) Determination of phosphate by differential spectrophotometry. Anal Chem 25: 1320
Klein RA (1970) The large scale preparation of unsaturated phosphatidyl cholines from egg yolk. Biophys Acta 219: 496
Porter NA, Weenen H (1981) High-performance liquid chromatographic separations of phospholipids and phospholipid oxidation products. Methods Enzymol 72: 34–40
Spanjer HH, Scherphof GL (1985) Targeting of liposomes to rat hepatocytes in vivo. Abstracts of the 13th International Congress of Biochemistry, Amsterdam. Elsevier Science, TU 380, p 326
Weber U, Goerz G (1986) Carotinoid-Retinopathie. III. Reversibilität. Klin Monatsbl Augenheilkd 188: 20–22
Weber U, Goerz G, Hennekes R (1985a) Carotinoid-Retinopathie. I. Morphologische und funktionelle Befunde bei 30 Carotinoidbehandelten Patienten. Klin Monatsbl Augenheilkd 187: 507–511
Weber U, Hennekes R, Goerz G (1985b) Carotinoid-Retinopathie. II. Elektrophysiologische Befunde bei 23 carotinoid-behandelten Patienten. Klin Monatsbl Augenheilkd 187: 507–511
Weber U, Kern W, Novotny GEK, Goerz G, Hanappel S (1987) Experimental carotenoid retinopathy. I. Functional and morphological alterations of the rabbit retina after 11 month's dietary carotenoid application. Graefe's Arch Clin Exp Ophthalmol 225: 198–205
About this article
Cite this article
Weber, U., Michaelis, L., Kern, W. et al. Experimental carotenoid retinopathy. Graefe's Arch Clin Exp Ophthalmol 225, 346–350 (1987). https://doi.org/10.1007/BF02153403
- Electron Microscopy
- Single Dose