(1) By means of a simple method the conditions were investigated for contraction and relaxation of actomyosin (AM) under the influence of potassium, magnesium, and calcium ions, of adenosintriphosphate (ATP) or muscle-adenylic-acid and phosphocreatin.
(2) Raising the calcium chloride concentration to 0.2 m and raising thep H from 6.9 to 7.3 hinders the contraction of actomyosin by ATP; in uncontracted AM-gel, ATP leads to a relaxation under these conditions.
(3) Relaxation of the contracted gel can be brought about by dissolving in potassium chloride and precipitating by dilution: calcium furthers this relaxation; afterward ATP leads again to a contraction.
(4) Remainders of ATP from the first contraction lead in this stage again to a partially contracted gel; phosphocreatin reinforces this effect.
(5) Certain SH-groups and amino groups, but not carboxyl groups, are involved in the production of the contraction.
(6) SH- and amino groups are also necessary for the ATP-ase effect. The relation of actomyosin-ATP-ase to contraction, of myosin-ATP-ase to relaxation is pointed out.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
H. H. Weber, Erg. Physiol.36, 109 (1933).
A. Szent-Györgyi,Chemistry of Muscular Contraction, Acad. Press., Inc., Publishers, New York, 1947, S. 73.
F. Buchthal, A. Deutsch, G. G. Knappeis undA. Munch-Petersen, Acta physiol. Scand.16, 326 (1949).
A. Szent-Györgyi, l.c., S. 36.
F. Buchthal, A. Deutsch undG. Knappeis, Nature153, 774 (1944).
M. Dubuisson, Exper.3, 213 (1947); Arch. int. Physiol.50, 203 (1940).
L. Hahn undG. Hevesy, Acta physiol. Scand.2, 51 (1941).
T. Noonan, W. Fenn, L. Haege, Amer. J. Physiol.133, 254 (1941).
T. P. Singer undE. S. G. Barron, Proc. Soc. Exper. Biol. Med.56, 120 (1944).
K. Bailey undS. V. Perry, Biochem. et biophys. acta1, 506 (1947).
R. M. Herriot, M. L. Anson undJ. H. Northrop, J. gen. Physiol.30, 187 (1946).
A. Szent-Györgyi, l.c., und zwar S. 55.
About this article
Cite this article
Kuschinsky, G., Turba, F. Über den Chemismus von Zustandsänderungen des Aktomyosins. Experientia 6, 103–106 (1950). https://doi.org/10.1007/BF02153375