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Modulation and selection of neurotransmitter responses during synapse formation between identified leech neurons

Summary

  1. 1.

    Serotonin (5-HT) modulates two different responses in the pressure sensitive neurons (P) of the leech: an inhibitory, Cl dependent synaptic response and a depolarizing extrasynaptic response.

  2. 2.

    Serotonergic Retzius cells (R)in vivo and in culture elicit inhibitory Cl dependent responses in P neurons. Moreover, at discrete sites of contact between R and P cells, the excitatory response to 5-HT is gradually lost prior to synapse formation. This phenomenon is specifically mediated by R cells.

  3. 3.

    The extrasynaptic response is mediated by cation channels sensitive to protein kinase C (PKC). Cation channels are present at the sites of contact but they become insensitive to PKC. Moreover, cation channels from single P cells are no longer modulated by PKC if they are inserted (by cramming the patch pipette) into the cytoplasm of a P cell in contact with an R cell.

  4. 4.

    Blockers of tyrosine kinases prevent the uncoupling of cation channel modulation and inhibit synapse formation between the R and the P neurons.

  5. 5.

    We suggest that cell contact induces an intracellular, tyrosine kinase-dependent signal as part of the mechanism of neuronal recognition leading to synapse formation.

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Catarsi, S., Drapeau, P. Modulation and selection of neurotransmitter responses during synapse formation between identified leech neurons. Cell Mol Neurobiol 16, 699–713 (1996). https://doi.org/10.1007/BF02151905

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Key words

  • synapse formation
  • leech neurons
  • serotonin
  • channel modulation