An ordinary Langmuir-trough for the spreading of proteins is divided into two equal spheres by a glass barrier (see fig. 1). Protein mono-layers can easily be transfered from the surface where they are spread to the adjacent surface, where the binding of dyestuffs (Evansblue, Trypan red, Trypan blue, Congo red, Naphthol yellow) takes place. The coloured protein mono-layer is brought into the condensed state (see figure 1a), deposited upon a glass slide (see figure 1b) and dissolved in 3 cm3 of 0.1-n-NaOH. Protein as well as dyestuffcontent are evaluated in the Beckman spectrophotometer at their respectively maximal absorption. The transfer of the mono-layer makes it impossible that protein which is dissolved in the substrate during the process of spreading, is measured additionally. This constitutes the first appliance for separating the surface for spreading and the surface where reactions of the surface-chemical type take place. From the extent of binding of the different dyestuffs by serum albumin and fibrinogen, it can be deduced that their dispersion is the determining factor, so that it is a colloid-chemical reaction which governs the affinity between protein monolayer and azodyestuff.
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Wunderly, C. Über die Farbstoffbindung durch monomolekulare Proteinschichten. Experientia 7, 296–297 (1951). https://doi.org/10.1007/BF02150547