Neurophysiology

, Volume 20, Issue 6, pp 559–564 | Cite as

Effects of microiontophoretically injected AMP and cAMP on calcium current in dialyzed Helix pomatia neurons

  • N. I. Kononenko
  • A. D. Shcherbatko
Article
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Abstract

The effects of injecting cells with adenosine monophosphate (AMP) and cyclic adenosine monophosphate (cAMP) on calcium current were investigated during intracellular dialysis ofHelixpomatia neurons. Microiontophoretically injected AMP was found to lead to reinstatement of calcium current following dialysis-induced “wash-out,” as well as considerable stabilization of this current with the extracellular medium at normal pH. Current-voltage relationship of the current would then undergo a 10 mV shift towards depolarization values. Perfusing the cell with a solution containing 10 mM AMP then produced a qualitatively identical effect. Injecting the neuron iontophoretically with cAMP led to a decline in the amplitude of calcium current under the same conditions. Neither raising the pH of the intracellular solution to 8.1 nor adding 4-aminopyridine in order to depress the hydrogen ion current produced a qualitative alteration in the effects of injecting AMP and cAMP on calcium current.

Keywords

Hydrogen Calcium Adenosine Adenosine Monophosphate Calcium Current 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • N. I. Kononenko
  • A. D. Shcherbatko

There are no affiliations available

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