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Analysis of nucleic acid derivatives at the subnanomolar level. II. Quantitative analysis of ribose derivatives by tritium labeling

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Zusammenfassung

Es wird eine Methode zur quantitativen Analyse von Ribosederivaten beschrieben, welche etwa fünftausendmal empfindlicher ist als die spektrophotometrischen Standardverfahren.

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Literatur

  1. 3

    K. Randerath, Analyt. Biochem.21, 480 (1967).

  2. 4

    J. X. Khym andW. E. Cohn, J. Am. chem. Soc.82, 6380 (1960).

  3. 5

    Abbreviations: AR, adenosine; CR, cytidine; UR, uridine; GR, guanosine; AR', CR', UR', GR', the corresponding nucleoside trialcohol derivatives obtained by periodate oxidation and borohydride reduction.

  4. 6

    C. F. Gordon andA. L. Wolfe, Analyt. Chem.32, 574 (1960).

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Additional information

This work was supported by grants-in-aid from the U.S. Atomic Energy Commission (No. AT(30-1)2643), the U.S. Public Health Service (No. CA 05018), and the American Cancer Society (No. E-51-J). This is publication No. 1325 of the Cancer Commission of Harvard University.

The first paper of this series3 described a procedure for detecting subnanomolar quantities of mono- and oligonucleotides on PEI-cellulose thin layers.

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Randerath, K., Randerath, E. Analysis of nucleic acid derivatives at the subnanomolar level. II. Quantitative analysis of ribose derivatives by tritium labeling. Experientia 24, 1192–1193 (1968). https://doi.org/10.1007/BF02146613

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Keywords

  • Methylene Blue
  • Nucleoside
  • Tritium
  • Plasmin
  • Photooxidation