Enzyme activities in the rabbit cornea during immunogenic keratitis

  • N. J. van Haeringen
  • A. Bolkova
  • J. A. Oosterhuis
  • J. L. van Delft
Article
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Abstract

In rabbits inflammation of the cornea was induced by intrastromal injection of horse serum. Between 2 and 4 weeks after injection, infiltration of the cornea with leukocytes and neovascularization could be observed. During this period, the rabbits were killed and their corneas analyzed for protein, alkaline phosphatase, acid phosphatase,N-acetyl-β-D-glucosaminidase, and lactate dehydrogenase. The enzyme activities in the inflamed corneal stroma reflect the high lysosomal activity, which probably originates from the leukocytes. The enzyme activities in the epithelium indicate that the tissue is abnormal and undergoing repair processes.

Keywords

Public Health Enzyme Enzyme Activity Lactate Alkaline Phosphatase 
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References

  1. Bolkova A (1977) Biochemical study on some acid hydrolases in the normal rabbit eye. Graefe's Arch Clin Exp Ophthalmol 203:67–71CrossRefGoogle Scholar
  2. Bolkova A, Obenberger J (1976) Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury. Graefe's Arch Clin Exp Ophthalmol 200:251–255CrossRefGoogle Scholar
  3. Breebaart AC (1960) Experimental corneal anaphylaxis. Thesis, University of Amsterdam, The NetherlandsGoogle Scholar
  4. Cejkova J, Bolkova A (1977) A study on alkaline phosphatase in cornea of various animals with special regards to keratocytes. Graefe's Arch Clin Exp Ophthalmol 204:209–214CrossRefGoogle Scholar
  5. Chayakul V, Reim M (1982) Enzymatic activity of β-N-acetylglucosaminidase in the alkali-burned rabbit cornea. Graefe's Arch Clin Exp Opthalmol 218:149–152Google Scholar
  6. Kaplunovich PS, Eremina II, Kolina MB, Skryabina GN (1973a) Alkaline phosphatase and inorganic phosphorus content in the cornea following chemical burns. Vestn Ofthalmol 6:40–42Google Scholar
  7. Lowry OR, Rosenborough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  8. Morawiecki J (1956) Präzipitationserscheinungen in der lebenden Hornhaut bei Antigenantikörperreaktionen. Ophthalmilogica 132:236–242Google Scholar
  9. Raekallio J (1970) Enzyme histochemistry of wound healing. Prog. Histochem Cytochem 1: 51–152Google Scholar
  10. Smolin G, Okumoto M, Hall J, Friedlaender M (1979) The effect of indoxole on the corneal immunologic response. Ann Ophthalmol 11:227–232PubMedGoogle Scholar
  11. Van Haeringen NJ; Oosterhuis JA, Van Delft JL, Barthen ER (1983) Inhibitors of prostaglandin synthesis and the cornea. Doc Ophthalmol 56:23–25CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • N. J. van Haeringen
    • 1
  • A. Bolkova
    • 2
  • J. A. Oosterhuis
    • 3
  • J. L. van Delft
    • 3
  1. 1.Biochemical LaboratoryThe Netherlands Ophthalmic Research InstituteAmsterdamThe Netherlands
  2. 2.Institute of Experimental MedicineCzechoslovac Academy of SciencesPrahaCzechoslovakia
  3. 3.Department of OphthalmologyLeiden Medical CenterLeidenThe Netherlands

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