Agents and Actions

, Volume 23, Issue 3–4, pp 153–156 | Cite as

Anti-allergic constituents in the culture medium ofGanoderma lucidum. (I) Inhibitory effect of oleic acid on histamine release

  • K. Tasaka
  • M. Akagi
  • K. Miyoshi
  • M. Mio
  • T. Makino
Histamine Release

Abstract

The chloroform extract fromGanoderma lucidum broth markedly inhibited histamine release from rat peritoneal mast cells. From the active fractions, palmitic acid, stearic acid, oleic acid and linoleic acid were isolated. Oleic acid dose-dependently inhibited the histamine release and45Ca uptake into mast cells induced by compound 48/80 and A-23187 at concentrations of 5 to 50 μM and 0.5 to 5 μM, respectively. Saturated fatty acids, however, had only a weak inhibitory effect on histamine release. Although linoleic acid and linolenic acid effectively prevented this release, these two compounds caused marked release at concentrations higher than 10 μM and 20 μM, respectively. Oleic acid induces membrane-stabilization in model membrane systems. It was concluded that one of the effective constituents obtainable from the chloroform extract ofG. lucidum-cultured broth is oleic acid.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    H. Kohda, W. Tokumoto, K. Sakamoto, M. Fujii, Y. Hirai, K. Yamasaki, Y. Komoda, H. Nakamura, S. Ishihara and M. Uchida,The biologically active constituents of Ganoderma lucidum (Fr.) Karst.Histamine release-inhibitory triterpenes, Chem. Pharm. Bull.33, 1367–1374 (1985).PubMedGoogle Scholar
  2. [2]
    K. Tasaka, M. Akagi and K. Miyohsi,Distribution of actinfilaments in rat mast cells and its role in histamine release, Agents and Action18, 49–52 (1986).CrossRefGoogle Scholar
  3. [3]
    P. A. Shore,Fluorometric assay of histamine. In:Methods in Enzymology, Vol. 17/b pp. 842–845 (Ed. H. Tabor and C. W. Tabor). Academic Press, New York 1971.Google Scholar
  4. [4]
    M. Mio, A. Ikeda, M. Akagi and K. Tasaka,Inhibitory effect of lysophosphatidylcholine on the histamine release from rat peritoneal mast cells, Agents and Actions16, 113–117 (1985).CrossRefPubMedGoogle Scholar
  5. [5]
    M. Akagi, M. Mio and K. Tasaka,Histamine release inhibition and prevention of the decrease in membrane fluidity induced by certain antiallergic drugs: analysis of the inhibitory mechanism of NCO-650, Agents and Action13, 149–156 (1983).CrossRefGoogle Scholar
  6. [6]
    M. Akagi, M. Mio, K. Tasaka and S. Kiniwa,Mechanism of histamine release inhibition induced by azelastine, Pharmacometrics26, 191–198 (1983).Google Scholar
  7. [7]
    C. E. Creutz,Cis-unsaturated fatty acids induce the fusion of chromaffin granules aggregated by synexin, J. Cell Biol.91, 247–256 (1981).CrossRefPubMedGoogle Scholar
  8. [8]
    A. Seelig and J. Seelig,Effect of a single cis double bond on the structure of a phospholipid bilayer, Biochemistry16, 45–50 (1977).CrossRefPubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1988

Authors and Affiliations

  • K. Tasaka
    • 1
  • M. Akagi
    • 1
  • K. Miyoshi
    • 1
  • M. Mio
    • 1
  • T. Makino
    • 2
  1. 1.Department of Pharmacology, Faculty of Pharmaceutical SciencesOkayama UniversityOkayamaJapan
  2. 2.Sunstar Research CenterTakatsukiJapan

Personalised recommendations