A method for isolating fluorescein glucuronide from the urine using silica gel chromatography and preparative high-performance liquid chromatography is described. The product obtained was free from significant fluorescent impurity on thin-layer chromatography and analytical high-performance liquid chromatography.
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Sakai, Y., Seto, C. & Araie, M. Study of fluorescein glucuronide. Graefe's Arch Clin Exp Ophthalmol 224, 110–112 (1986). https://doi.org/10.1007/BF02141480
- Public Health
- Liquid Chromatography
- Fluorescent Impurity