Lasers in Medical Science

, Volume 10, Issue 1, pp 43–46 | Cite as

Photodynamic therapy of experimental tumours with Zn(II)-phthalocyanine and pulsed laser irradiation

  • M. Shopova
  • N. Stoichkov
  • A. Milev
  • M. Peev
  • K. Georgiev
  • A. Gizbreht
  • G. Jori
  • F. Ricchelli
Original Articles
Received:
Revised:

Abstract

The effectiveness of a pulsed dye laser (673 nm) for photodynamic therapy (PDT) of tumours in the presence of Zn(II)-phthalocyanine (ZnPc) was evaluated using Lewis lung carcinoma-bearing mice. The tumours were irradiated with different pulse energies (from 0.4 to 10 mJ) at a constant fluence of 0.6 J cm−2 at 24 h after administration of 0.25 mg kg−1 body weight liposome-incorporated ZnPc. Maximal PDT effect, as evaluated by changes in mean tumour diameter, animal survival time and histological evaluation of tumour necrosis, was observed after 3.0 mJ pulse energy irradiation which appears to yield a deeper light penetration and a more efficient sensitizer excitation when compared with lower or higher pulse energies. Electron microscopic analysis of photo-treated tumour indicates preferential damage to malignant tissue as compared to endothelial cells.

Key words

Lewis lung carcinoma Liposomes Photodynamic therapy Pulsed laser irradiation Zn(II)-phthalocyanine 
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Copyright information

© W.B. Saunders Company Ltd 1995

Authors and Affiliations

  • M. Shopova
    • 1
  • N. Stoichkov
    • 2
  • A. Milev
    • 2
  • M. Peev
    • 1
  • K. Georgiev
    • 1
  • A. Gizbreht
    • 3
  • G. Jori
    • 4
  • F. Ricchelli
    • 4
  1. 1.Institute of Organic ChemistryBulgarian Academy of SciencesSofiaBulgaria
  2. 2.National Center of OncologySofiaBulgaria
  3. 3.Institute of ElectronicsBulgarian Academy of SciencesSofiaBulgaria
  4. 4.C.N.R. Centre of Metalloproteins and Department of BiologyUniversity of PadovaPadovaItaly

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