Lymphotropic papovaviruses isolated from African green monkey and human cells
- 53 Downloads
A lymphotropic papovavirus was isolated from a lymphoblastoid cell line of African green monkey (AGM) cells which also contained a herpesvirus and a paramyxovirus-like agent. The papovavirus was analyzed by restriction endonuclease cleavage; its biochemical and serological crossreactivity with SV40 and host range have been determined. Thus far, only B-lymphoblasts of primate and human origin have been found to be susceptible to infection. Although more than 50% of the tested monkey sera were reactive with antigens of this virus, all human sera tested failed to react. Cleavage patterns and hybridization studies with the viral DNA indicate that the virus represents a novel member of the papovavirus group that is characterized by its lymphotropic host range. Papovavirus particles were also demonstrated in a human lymphoblastoid cell line (CCRF-SB) originally derived from a leukemic child. These cells revealed nuclear fluorescence when tested with human sera, but failed to react with AGM sera. Although characterization of this agent has not yet been completed, available evidence suggests that it represents another lymphotropic papovavirus which seems to be spread within the human population.
Unable to display preview. Download preview PDF.
- Foley, G.E., Lazarus, H., Farber, S., Uzman, B.G., Adams, R.A.: Studies on human leukemic cells in vitro. In: The proliferation and spread of neoplastic cells. 21st Annual Symposium on Fundamental Cancer Research, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX. pp. 65–94. Baltimore. The Williams & Wilkins Co., 1968Google Scholar
- Hinuma, Y., Konn, M., Yamaguchi, J. Wudarski, D.J., Blakeslee, J.R., Grace, J.T.: Immunofluorescence and herpes-type particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol.1, 1045–1051 (1976)Google Scholar
- Iwakata, S., Grace, J.T.: Cultivation in vitro of myeloblasts from human leukemia. N.Y. State Med. J.64, 2279–2282 (1964)Google Scholar
- Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menézes, J., Nilsson, K., Sundström, Ch.: Continuous lymphoid cell lines with characteristics of B cells (bone-marrowderived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. Natl. Acad. Sci. USA71, 3283–3286 (1974)PubMedGoogle Scholar
- Klein, G., Giovanella, B., Westman, A., Stehlin, J.S., Munford, D.: An EBV-Genomenegative cell line established from an American Burkitt lymphoma; receptor characteristics, EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology5, 319–334 (1975)PubMedGoogle Scholar
- Lazarus, H., Barell, E.F., Oppenheim, S., Krishnan, A.: Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood. In Vitro9, 303–310 (1974)Google Scholar
- Schachat, F.H., Hogness, D.S.: Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. CSHSQB38, 371–381 (1973)Google Scholar