Medical Microbiology and Immunology

, Volume 167, Issue 3, pp 137–153

Lymphotropic papovaviruses isolated from African green monkey and human cells

  • Harald zur Hausen
  • Lutz Gissmann


A lymphotropic papovavirus was isolated from a lymphoblastoid cell line of African green monkey (AGM) cells which also contained a herpesvirus and a paramyxovirus-like agent. The papovavirus was analyzed by restriction endonuclease cleavage; its biochemical and serological crossreactivity with SV40 and host range have been determined. Thus far, only B-lymphoblasts of primate and human origin have been found to be susceptible to infection. Although more than 50% of the tested monkey sera were reactive with antigens of this virus, all human sera tested failed to react. Cleavage patterns and hybridization studies with the viral DNA indicate that the virus represents a novel member of the papovavirus group that is characterized by its lymphotropic host range. Papovavirus particles were also demonstrated in a human lymphoblastoid cell line (CCRF-SB) originally derived from a leukemic child. These cells revealed nuclear fluorescence when tested with human sera, but failed to react with AGM sera. Although characterization of this agent has not yet been completed, available evidence suggests that it represents another lymphotropic papovavirus which seems to be spread within the human population.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Carroll, R.B., Smith, A.E.: Monomer molecular weight of T-antigen from simian virus 40-infected and -transformed cells. Proc. Natl. Acad. Sci. USA73, 2254–2258 (1976)PubMedGoogle Scholar
  2. Dugaiczyk, A. Boyer, H.W., Goodman, H.M.: Digestion of Eco RI endonucleasegenerated DNA fragments into linear and circular structures. J. Mol. Biol.96, 171–184 (1975)CrossRefPubMedGoogle Scholar
  3. Falk, L.A., Henle, G., Henle, W., Deinhardt, F., Schudel, A.: Transformation of lymphocytes by Herpesvirus Papio. Int. J. Cancer20, 219–226 (1977)PubMedGoogle Scholar
  4. Fareed, G.E., Davoli, D.: Molecular biology of papovaviruses. Ann. Rev. Biochem.46, 471–522 (1977)CrossRefPubMedGoogle Scholar
  5. Fiers, W., Contreras, R., Haegeman, G., Rogiers, R., Van de Voorde, A., Van Heuverswyn, H., Van Herreweghe, J., Volckaert, G., Ysebaert, M.: Complete nucleotide sequence of SV 40 DNA. Nature273, 113–120 (1978)CrossRefPubMedGoogle Scholar
  6. Foley, G.E., Lazarus, H., Farber, S., Uzman, B.G., Adams, R.A.: Studies on human leukemic cells in vitro. In: The proliferation and spread of neoplastic cells. 21st Annual Symposium on Fundamental Cancer Research, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX. pp. 65–94. Baltimore. The Williams & Wilkins Co., 1968Google Scholar
  7. Fresen, K.O., zur Hausen, H.: Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV) — genome-negative human lymphoma cells with different EBV strains. Int. J. Cancer17, 161–166 (1976)PubMedGoogle Scholar
  8. Fresen, K.O., Merkt, B., Bornkamm, G.W., zur Hausen, H.: Heterogeneity of Epstein-Barr Virus originating from P3HR-1 cells. I. Studies on EBNA induction. Int. J. Cancer19, 317–323(1977)PubMedGoogle Scholar
  9. Friedman, T., Doolittle, R.F., Walter, G.: Amino acid sequence homology between polyoma and SV 40 tumor antigens deduced from nucleotide sequences. Nature274, 291–292 (1978)CrossRefPubMedGoogle Scholar
  10. Gardner, S.D., Field, A.M., Coleman, D.V., Hulme, B.: New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet,1971 I, 1253–1257CrossRefGoogle Scholar
  11. Gibson, W.: Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology62, 391–336 (1974)CrossRefGoogle Scholar
  12. Gissmann, L., zur Hausen, H.: Human papilloma viruses: physical mapping and genetic heterogeneity. Proc. Natl. Acad. Sci. USA73, 1310–1313 (1976)PubMedGoogle Scholar
  13. Gissmann, L., Pfister, H., zur Hausen, H.: Human papillomaviruses (HPV): characterization of four different isolates. Virology76, 569–580 (1977)CrossRefPubMedGoogle Scholar
  14. Harrison, T., Graham, S., Williams, J.: Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology77, 319–329 (1977)CrossRefPubMedGoogle Scholar
  15. Henle, G., Henle, W.: Immunofluorescence in cells derived from Burkitt's lymphoma. J. Bact.91, 1248–1256 (1966)PubMedGoogle Scholar
  16. Hinuma, Y., Konn, M., Yamaguchi, J. Wudarski, D.J., Blakeslee, J.R., Grace, J.T.: Immunofluorescence and herpes-type particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol.1, 1045–1051 (1976)Google Scholar
  17. Iwakata, S., Grace, J.T.: Cultivation in vitro of myeloblasts from human leukemia. N.Y. State Med. J.64, 2279–2282 (1964)Google Scholar
  18. Klein, G., Lindahl, T., Jondal, M., Leibold, W., Menézes, J., Nilsson, K., Sundström, Ch.: Continuous lymphoid cell lines with characteristics of B cells (bone-marrowderived), lacking the Epstein-Barr virus genome and derived from three human lymphomas. Proc. Natl. Acad. Sci. USA71, 3283–3286 (1974)PubMedGoogle Scholar
  19. Klein, G., Giovanella, B., Westman, A., Stehlin, J.S., Munford, D.: An EBV-Genomenegative cell line established from an American Burkitt lymphoma; receptor characteristics, EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology5, 319–334 (1975)PubMedGoogle Scholar
  20. Lazarus, H., Barell, E.F., Oppenheim, S., Krishnan, A.: Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood. In Vitro9, 303–310 (1974)Google Scholar
  21. Minowada, J., Ohnuma, T., Moore, G.E.: Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst.49, 891–895 (1972)PubMedGoogle Scholar
  22. Padgett, B.L., ZuRhein, G.M., Walker, D.L., Eckrade, R.J.: Cultivation of papovalike virus from human brain with progressive multifocal leukoencephalopathy. Lancet1971 I, 1257–1260CrossRefGoogle Scholar
  23. Pulvertaft, R.T.V.: A study of malignant tumors in Nigeria by short term tissue culture. J. Clin. Path.18, 261–273 (1965)PubMedGoogle Scholar
  24. Schachat, F.H., Hogness, D.S.: Repetitive sequences in isolated Thomas circles from Drosophila melanogaster. CSHSQB38, 371–381 (1973)Google Scholar
  25. Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975)PubMedGoogle Scholar
  26. Tai, H.R., Smith, C.A., Sharp, P.A., Vinograd, J.: Sequence heterogeneity in closed circular simian virus 40 deoxyribonucleic acid. J. Virol.9, 317–315 (1972)PubMedGoogle Scholar
  27. Waldeck, W., Sauer, G.: New oncogenic papova virus from primate cells. Nature269, 171–173 (1977)CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Harald zur Hausen
    • 1
  • Lutz Gissmann
    • 1
  1. 1.Institut für Virologie, Zentrum für HygieneUniversitÄt FreiburgFreiburgGermany

Personalised recommendations