Medical Microbiology and Immunology

, Volume 166, Issue 1–4, pp 177–186 | Cite as

Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques

  • Maureen G. Friedman
  • Hava Haikin
  • Sophia Leventon-Kriss
  • Raya Joffe
  • Varda Goldstein
  • Israel Sarov

Abstract

An enzyme assay for the detection of antibodies to varicella-zoster membrane antigen (IPAMA) and a solid phase radioimmunoassay (RIA) which utilizes infected cell lysates as antigen are described. The results have been compared with those obtained by the indirect fluorescent antibody to membrane antigen (IFAMA) technique.

It was found that IPAMA was equal in sensitivity to IFAMA. No cross-reactivity was found with other herpes group viruses. The IPAMA appears to give objective results, is easily and rapidly performed, and is recommended as a routine test for serological diagnosis of varicella-zoster infection.

The RIA method is about 100 times more sensitive than IPAMA and IFAMA. The RIA is specific and has the potential of determining lower levels of antibody than other serological methods currently in use.

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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Maureen G. Friedman
    • 1
  • Hava Haikin
    • 1
  • Sophia Leventon-Kriss
    • 2
  • Raya Joffe
    • 2
  • Varda Goldstein
    • 1
  • Israel Sarov
    • 1
  1. 1.Virology Unit, Faculty of Health Sciences, and Soroka Medical CenterBen-Gurion University of the NegevBeer-ShevaIsrael
  2. 2.Central Virology LaboratoryMinistry of HealthTel-Aviv-JaffaIsrael

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