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Medical Microbiology and Immunology

, Volume 157, Issue 4, pp 346–356 | Cite as

Der Plaquereduktionstest zur Bestimmung der virusneutralisierenden Potenz eines Serums

  • A. Grafe
Article
  • 67 Downloads

Zusammenfassung

Im Plaquetest kommt man bei Anwendung bestimmter graphischer Auswerteverfahren zur gleichen Bewertung der virusneutralisierenden Potenz eines Serums wie im Röhrchentest, bei dem mit 100 TCID50 im Virus/Serumgemisch gearbeitet wird. Trägt man die mit verschiedenen Verdünnungen eines Serums gewonnenen Plaquemittelwerte im halblogarithmischen Netz auf, so läßt sich eine Kurve konstruieren, deren Schnittpunkt mit der Abszisse den Wert der völligen Plaquehemmung angibt, der mit dem errechneten Antikörpertiter des Röhrchentestes übereinstimmt.

Im Wahrscheinlichkeitsnetz, in dem es eine Bestimmung des 100%igen Plaque-reduktionswertes nicht geben kann, erreicht man mit der Feststellung des Wertes der 90%igen Plaquereduktion eine Serumverdünnung, die nur geringfügig vom im Röhrchentest errechneten Antikörpertiter abweicht.

Zur besseren Überschaubarkeit und Vergleichbarkeit von in verschiedenen Labors erzielten Testergebnissen wird die Bestimmung dieses 90%igen Plaquereduktionswertes allen anderen Reduktionswerten vorgezogen.

Plaque assay for determining the virus-neutralizing potency of serum

Summary

The results in a plaque assay are the same as in a test with tubes, in which 100 TCID50 is used in the virus-serum-mixture on the understanding that the plaque assay is evaluated by a distinct graphic method. If the mean plaque values obtained by different dilutions of a serum are plotted in a half-logarithmic net, the point of intersection between the constructed curve and the abscissa is equal to the titer obtained in test tubes.

In the probability net, in which the fixing of 100% plaque reduction is impossible, the value of a 90% plaque reduction differs very little from the calculated antibody titer in test tubes.

The destination of the 90% plaque reduction is to be preferred to all other methods in the plaque reduction neutralization test.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • A. Grafe
    • 1
  1. 1.Medizinische ForschungBoehringer Mannheim GmbHDeutschland

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