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Zeitschrift für Rechtsmedizin

, Volume 90, Issue 4, pp 239–246 | Cite as

Combined ulcerogenic effect of ethanol and acetylsalicylic acid on the gastric mucosa of the rat

  • J. Hirvonen
  • P. Huttunen
  • K. -P. Martimo
  • J. Puurunen
Originalarbeiten / Original Works

Summary

The effect on rat gastric mucosa of ethanol and acetylsalicylic acid given at two doses singly or in combination were studied by administering the drugs into the stomach. 40% ethanol alone caused many ulcerations after 5 h, and the effect was even more marked with simultaneous administration of acetylsalicylic acid (ASA). 10% ethanol and 50 mg ASA/kg b.wt. were tolerated well. The mucosal lesions consisted of coagulation necrosis and hemorrhages, with a disappearance of histamine and serotonin from the mucosal mast cells in and around the lesion.

Key words

Gastric ulcers Acethylsalicylic acid Ethanol Histamine Serotonin 

Zusammenfassung

Die Wirkung von Ethanol, Acetylsalicylsäure (ASS) und deren Kombination auf die Magenschleimhaut wurde bei Ratten nach einer akuten, oralen Zufuhr untersucht. Fünf Stunden nach der Eingabe von 40%igem Ethanol wurden mehrere Schleimhautlasionen beobachtet, und diese Wirkung wurde bei einer gleichzeitigen Zufuhr von 50 und 200 mg ASS/kg verstärkt. Die Kombination von 10%igem Ethanol und 50 mg ASS/kg verursachte keine deutliche Schäden an der Magenschleimhaut. Die Schleimhautläsionen bestanden aus Koagulationsnekrosen und Hämorrhagien. Eine Verminderung von Histamin und Serotonin wurde in den Läsionen und deren Umgebung beobachtet.

Schlüsselwörter

Magengeschwür, durch Ethanol und Acetylsalicylsäure Histamin Serotonin 

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References

  1. Anton A, Sayre D (1969) A modified fluorometric procedure for tissue histamine and its distribution in various animals. J Pharmacol Exp Therap 166: 285–292Google Scholar
  2. Curzon G, Green A (1970) Rapid method for the determination of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid in small regions of rats brain. Br J Pharmacol 39: 653–655Google Scholar
  3. Davenport H (1975) The gastric mucosal barrier. Past, present, and future. Mayo Clin Proc 50: 507–514Google Scholar
  4. Hirvonen J, Penttilä A (1970) Staining and electron-microscopic characteristics of amine-storing cells in rat's gastric mucosa. Scand J Gastroenterol 5: 177–185Google Scholar
  5. Hirvonen J, Elfving R (1974) Histamine and serotonin in the gastric erosions of rats dead from exposure to cold: A histochemical and quantitative study. Z Rechtsmed 74: 273–281Google Scholar
  6. Håkanson R (1970) New aspects of the formation and function of histamine, 5-hydroxy- tryptamine and dopamine in gastric mucosa. Acta Physiol Scand [Suppl] 340: 1–134Google Scholar
  7. Levine R, Senay E (1968) Histamine in the pathogenesis of stress ulcers in the rat. Am J Physiol 214: 892–896Google Scholar
  8. Pearse AGE (1972) Histochemistry. Theoretical and applied, vol II, 3rd edn. Churchill, London, pp 1101–1127Google Scholar
  9. Penttilä A, Alfthan O, Hirvonen J (1969) Fluorescence and ultrastructural features of amine-storing cells in the human gastric mucosa. Ann Med Exp Fenn 47: 275–284Google Scholar
  10. Puurunen J, Huttunen P, Hirvonen J (1980) Is ethanol-induced damage of the gastric mucosa a hyperosmotic effect? Comparative studies on the effects of ethanol, some other hyperosmotic solutions and acetylsalicylic acid on rat gastric mucosa. Acta Pharmacol Toxicol 47: 321–327Google Scholar
  11. Watt J (1959) The mechanism of histamine ulceration in the guinea pig. Gastroenterology 37: 741–759Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • J. Hirvonen
    • 1
  • P. Huttunen
    • 1
  • K. -P. Martimo
    • 1
  • J. Puurunen
    • 1
  1. 1.Depts. of Forensic Medicine and PharmacologyUniversity of OuluOuluFinland

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