Immunogenetics

, Volume 32, Issue 3, pp 183–186

The human genes for the α and γ subunits of the mast cell receptor for immunoglobulin E are located on human chromosome band 1823

  • Maryvonne Le Coniat
  • Jean-Pierre Kinet
  • Roland Berger
Original Articles

Abstract

The receptor with high affinity for immunoglobulin E (FcERI) is a key molecule in triggering the allergic reaction. It isα tetrameric complex of oneα subunit, oneβ subunit, and two disulfide-linkedγ subunits. This receptor is present exclusively on mast cells and basophils. Molecules identical to theγ subunit of FcεRI also formα cell surface complex with other Fc receptors such as mouse FcγRIIa in macrophages and most probably with human FcγRIII (CD16) in natural killer (NK) cells. Here we show by in situ hybridization that the human genes for theα (FCER1A) and γ subunits (FCER1 G) of FcERI and the gene for FcγRIII (FCGR3, CD16) are located on human chromosome band 1823.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Blank, U., Ra., C., Miller, L., White, K., Metzger, H., and Kinet, J. P.: Complete structure and expression in transfected cells of high affinity IgE receptor.Nature 337: 187–189, 1989PubMedGoogle Scholar
  2. Caubet, J. F., Mathieu-Mahul, D., Bernheim, A., Larsen, C. J., and Berger, R.: Human proto-oncogene c-mos maps to 8g11.EMBO J 4: 2245–2248, 1985PubMedGoogle Scholar
  3. Feinberg, A. P. and Vogelstein, B.: A technique for radiolabeling DNA restriction endonuclease fragment to high specific activity.Anal Biochem 132: 6–13, 1983PubMedGoogle Scholar
  4. Grundy, H. O., Peltz, G. A., Moore, K. W., Golbus, M. S., Jackson, L. G., and Lebo, R. V.: The polymorphicFcγ receptor II gene maps to human chromosome 1q.Immunogenetics 29: 331–339, 1989PubMedGoogle Scholar
  5. Harper, M. E. and Saunders, G. F.: Localization ofα single copy DNA sequences on G-banded chromosomes by in situ hybridization.Chromosoma 83: 431–439, 1981PubMedGoogle Scholar
  6. Hibbs, M. L., Selvaraj, P., Carpen, O., Springer, T. A., Küster, H., Jouvin, M.-H. E., and Kinet, J. P.: Mechanisms for regulating expression of membrane isoforms of FcγRIII (CD16).Science, in press, 1990Google Scholar
  7. Huppi, K., Siwarski, D., Mock, B. A., and Kinet, J. P.: Gene mapping of the three subunits of the high affinity Fc receptor for IgE.J Immunol 143: 3787–3791, 1989PubMedGoogle Scholar
  8. Kinet, J. P.: Antibody-cell interactions: Fc receptors.Cell 57: 351–354, 1989PubMedGoogle Scholar
  9. Kochan, J., Pettine, L. F., Hakimi, J., Kishi, K., and Kinet, J. P.: Isolation of the gene coding for the alpha subunit of the human high affinity IgE receptor.Nucleic Acids Res 16: 3584, 1988PubMedGoogle Scholar
  10. Küster, H., Thompson, H., and Kinet, J. P.: Characterization and expression of the gene for the human Fc receptorγ subunit.J Biol Chem 265: 6448–6452, 1990PubMedGoogle Scholar
  11. Lanier, L. L., Yu, G., and Phillips, J. H.: Co-association of CD3ζ withα receptor (CD 16) for IgG Fc on human natural killer cells.Nature 342: 803–804, 1989PubMedGoogle Scholar
  12. Peltz, G. A., Grundy, H. O., Lebo, R. V., Yssel, H., Barsh, G. S., and Moore, K. W.: Human FcγRIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.Proc Natl Acad Sci USA 86: 1013–1017, 1989PubMedGoogle Scholar
  13. Ra, C., Jouvin, M. -H. E., Blank, U., and Kinet, J. P.: A macrophage Fcy receptor and the mast cell for IgE share an identical subunit.Nature 341: 752–754, 1989PubMedGoogle Scholar
  14. Ravetch, J. V. and Perussia, B.: Alternative membrane forms of FcγRIII (CD16) on human NK cells and neutrophils: cell-type specific expression of two genes which differ in single nucleotide substitutions.J Exp Med 170: 481–497, 1989PubMedGoogle Scholar
  15. Sammartino, L., Webber, L. M., Hogarth, P. M., McKenzie, I. F. C., and Garson, O. M.: Assignment of the gene coding for human FcRII (CD32) to bands g23g24 on chromosome 1.Immunogenetics 28: 380–381, 1988PubMedGoogle Scholar
  16. Tepler, I., Morton, C. C., Shimizu, A., Holcombe, R. F., Eddy, R., Shows, T. B., and Leder, P.: The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to lg21–q23 and RFLP analysis.Am J Hum Genet 45: 761–765, 1989PubMedGoogle Scholar
  17. Weissman, A. M., Hou, D., Orloff, D. G., Modi, W. S., Seuanez, H., O'Brien, S. J., and Klausner, R. D.: Molecular cloning and chromosomal localization of the human T-cell receptorζ chain: distinction from the CD3 complex.Proc Natl Acad Sci USA 85: 9709–9713, 1989Google Scholar
  18. Yunis, J. J., Sawyer, J. R., and Ball, D. W.: Characterization of metaphase-prophase G-banded chromosomes and their use in gene mapping.Cytogenet Cell Genet 22: 679–683, 1978PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Maryvonne Le Coniat
    • 1
  • Jean-Pierre Kinet
    • 2
  • Roland Berger
    • 1
  1. 1.Unité INSERM U 301 and SDI No. 15954 I CNRSInstitut de Génétique MoléculaireParisFrance
  2. 2.Molecular Allergy and Immunology SectionNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA

Personalised recommendations