Polymerase chain reaction for detection ofBorrelia burgdorferi DNA in skin lesions of early and late Lyme borreliosis
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The aim of this study was to evaluate the polymerase chain reaction (PCR) as a diagnostic tool for Lyme borreliosis on large numbers of samples from clinically well-defined cases of early and late cutaneous borreliosis. Skin biopsy specimens from patients with erythema migrans and acrodermatitis chronica atrophicans were analysed blindly together with an equal number of control biopsies. Using two different dilutions of each DNA specimen increased the number of total positives detected. All of the 76 control biopsies were PCR negative. Biopsy specimens from 18 of 26 (69 %) erythema migrans lesions and from 22 of 36 (61 %) acrodermatitis chronica atrophicans lesions were PCR positive. Fourteen post-therapy biopsies from patients with acrodermatitis chronica atrophicans were all negative, supporting the opinion that antibiotic therapy is successful in this chronic manifestation of Lyme borreliosis.
KeywordsPolymerase Chain Reaction Internal Medicine Diagnostic Tool Antibiotic Therapy Skin Lesion
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- 1.Jaulhac B, Nicolini P, Piemont Y, Monteil H: Detection ofBorrelia burgdorferi in cerebrospinal fluid of patients with Lyme borreliosis. New England Journal of Medicine 1991, 234: 1440.Google Scholar
- 4.Keller TL, Halperin JJ, Whitman M: PCR detection ofBorrelia burgdorferi DNA in cerebrospinal fluid of Lyme neuroborreliosis patients. Neurology 1992, 42: 32–42.Google Scholar
- 6.Amouriaux P, Assous M, Margarita D, Baranton G, Saint Giron I: Polymerase chain reaction with the 30-kb circular plasmid ofBorrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid. Research in Microbiology 1993, 144: 211–219.CrossRefPubMedGoogle Scholar
- 10.Schwartz I, Wormser GP, Schwartz JJ, Cooper D, Weissensee P, Gazumyan A, Zimmerman E, Goldberg NS, Bittker S, Campbell GL, Pavia CS: Diagnosis of early Lyme disease by polymerase chain reaction amplification and culture of skin biopsies from erythema migrans lesions. Journal of Clinical Microbiology 1992, 30: 3082–3088.PubMedGoogle Scholar
- 15.Åsbrink E: Cutaneous manifestations of Lyme borreliosis: clinical definitions and differential diagnosis. Scandinavian Journal of Infectious Diseases 1991, Supplement 77: 44–50.Google Scholar
- 16.Baranton G, Postic D, Saint Girons I, Boerlin P, Piffaretti JC, Assous M, Grimont PAD: Delineation ofBorrelia burgdorferi sensu stricto,Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. International Journal of Systematic Bacteriology 1992, 42: 378–383.PubMedGoogle Scholar
- 20.Persing DH, Barthold SW, Malawista SE: Molecular detection ofBorrelia burgdorferi. Current Communications in Cell & Molecular Biology 1992, 6: 299–315.Google Scholar
- 21.Åsbrink E, Hovmark A: Successful cultivation of spirochetes from skin lesions of patients with erythema chronicum migrans afzelius and acrodermatitis chronica atrophicans. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1985, 93: 161–163.Google Scholar
- 25.Greenfield L, White TJ: Sample preparation methods. In: Persing DH, Smith TF, Tenover FC, White TJ (ed): Diagnostic molecular biology. Principles and applications. American Society for Microbiology, Washington DC, 1993, p. 122–137.Google Scholar
- 26.Philipson A: Antibiotic treatment in Lyme borreliosis. Scandinavian Journal of Infectious Diseases 1991, Supplement 7: 145–150.Google Scholar