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Production of siderophore by coagulase-negative staphylococci and its relation to virulence

  • J. A. Lindsay
  • T. V. Riley
  • B. J. Mee
Notes

Abstract

The ability to produce siderophore is considered to be a virulence factor for many pathogenic bacteria. To determine if siderophore production by coagulase-negative staphylococci (CNS) was related to virulence, 40 clinical isolates of CNS cultured from peritoneal dialysis fluid were compared with 38 commensal skin isolates. Siderophore activity was detected using the chrome azurol S liquid assay. Using precursor studies,Staphylococcus epidermidis isolates were shown to be more likely to produce the siderophore staphyloferrin A. Production of staphyloferrin B amongst non-Staphylococcus epidermidis species was associated with clinical isolates rather than commensal isolates, and therefore may play a role in pathogenicity.

Keywords

Internal Medicine Chrome Peritoneal Dialysis Virulence Factor Clinical Isolate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pfaller MA, Herwaldt LA Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clinical Microbiology Reviews 1988, 1: 281–299.PubMedGoogle Scholar
  2. 2.
    Kloos WE, Bannerman TL Update on clinical significance of coagulase-negative staphylococci. Clinical Microbiology Reviews 1994, 7: 117–140.PubMedGoogle Scholar
  3. 3.
    Sewell CM, Clarridge JE, Young EJ, Guthrie RK Clinical significance of coagulase-negative staphylococci. Journal of Clinical Microbiology 1982, 16: 236–239.PubMedGoogle Scholar
  4. 4.
    Hussain M, Wilcox MH, White PJ The slime of coagulase-negative staphylococci: biochemistry and relation to adherence. FEMS Microbiology Reviews 1993, 104: 191–208.Google Scholar
  5. 5.
    Wooldridge KG, Williams PH Iron uptake mechanisms of pathogenic bacteria. FEMS Microbiology Reviews 1993, 12: 325–348.PubMedGoogle Scholar
  6. 6.
    Lindsay JA, Riley TV Staphylococcal iron requirements, siderophore production, and iron-regulated protein expression. Infection and Immunity 1994, 62: 2309–2314.PubMedGoogle Scholar
  7. 7.
    Meiwes J, Fiedler H-P, Haag H, Zähner H, Konetschny-Rapp S, Jung G Isolation and characterization of staphyloferrin A, a compound with siderophore activity fromStaphylococcus hyicus DSM 20459. FEMS Microbiology Letters 1990, 67: 201–206.Google Scholar
  8. 8.
    Konetschny-Rapp S, Jung G, Meiwes J, Zähner H Staphyloferrin A: a structurally new siderophore from staphylococci. European Journal of Biochemistry 1990, 191: 65–74.PubMedGoogle Scholar
  9. 9.
    Drechsel H, Freund S, Nicholson G, Haag H, Jung O, Zähner H, Jung G Purification and chemical characterization of staphyloferrin B, a hydrophilic siderophore from staphylococci. BioMetals 1993, 6: 185–192.PubMedGoogle Scholar
  10. 10.
    Haag H, Fiedler HP, Meiwes J, Drechsel H, Jung G, Zähner H Isolation and biological characterization of staphyloferrin B, a compound with siderophore activity from staphylococci. FEMS Microbiology Letters 1994, 115: 125–130.PubMedGoogle Scholar
  11. 11.
    Lindsay JA, Riley TV Susceptibility to desferrioxamine: a new test for the identification ofStaphylococcus epidermidis. Journal of Medical Microbiology 1991, 35: 45–48.PubMedGoogle Scholar
  12. 12.
    Lindsay JA, Aravena-Román MA, Riley TV Identification ofStaphylococcus epidermidis andStaphylococcus hominis from blood cultures by testing susceptibility to desferrioxamine. European Journal of Clinical Microbiology & Infectious Diseases 1993, 12: 127–131.Google Scholar
  13. 13.
    Kloos WE Systematics and the natural history of staphylococci. Part I. Journal of Applied Bacteriology 1990, Supplement: 25–37.Google Scholar
  14. 14.
    Schwyn B, Neilands JB Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 1987, 160: 47–56.PubMedGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • J. A. Lindsay
    • 1
  • T. V. Riley
    • 2
  • B. J. Mee
    • 1
  1. 1.Department of MicrobiologyUniversity of Western Australia, Queen Elizabeth II Medical CentreNedlandsAustralia
  2. 2.Department of Clinical MicrobiologySir Charles Gairdner Hospital, Queen Elizabeth II Medical CentreNedlandsAustralia

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