Evaluation of a commercial rRNA target amplification assay for detection ofMycobacterium tuberculosis complex in respiratory specimens

  • S. Ehlers
  • M. Pirmann
  • W. Zaki
  • H. Hahn
Note

Abstract

Seventy-one respiratory samples were analyzed in a new commercially available target rRNA amplification assay aimed at detectingMycobacterium tuberculosis complex directly in patient specimens. The assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test) correctly identified 26 of 27 samples positive forMycobacterium tuberculosis and 43 of 44 samples negative forMycobacterium tuberculosis in culture. When appropriate controls are performed, the assay also performs well with other specimens, such as tissue biopsies, lymph node or bone marrow aspirates and pleural and ascitic exudates.

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References

  1. 1.
    Abe C, Hosojima S, Fukasawa Y, Kazumi Y, Tkahashi M, Hirano K, Mori T: Comparison of MB-Check, BACTEC, and egg-based media for recovery of mycobacteria. Journal of Clinical Microbiology 1992, 30: 878–881.Google Scholar
  2. 2.
    Isenberg HD, D'Amato RF, Heifets L, Murray PR, Scadamaglia M, Jacobs MC, Alperstein P, Niles A: Collaborative feasibility study of a biphasic system (Roche Septi-Check AFB) for rapid detection and isolation of mycobacteria. Journal of Clinical Microbiology 1991, 29: 1719–1722.Google Scholar
  3. 3.
    Middlebrook G, Reggiardo ZH, Tigerit WD: Automatable radiometric detection of growth ofMycobacterium tuberculosis in selective media. American Review of Respiratory Disease 1977, 115: 1066–1069.PubMedGoogle Scholar
  4. 4.
    Arnold LJ, Hammond PW, Wiese WA, Nelson, NC: Assay formats involving acridinium-ester-labeled DNA probes. Clinical Chemistry 1989, 35: 1588–1594.PubMedGoogle Scholar
  5. 5.
    Hance AJ, Grandchamp B, Levy-Frebualt V, Lecossier D, Rauzier J, Bocart D, Gicquel B: Detection and identification of mycobacteria by amplification of mycobacterial DNA. Molecular Microbiology 1989, 3: 843–849.PubMedGoogle Scholar
  6. 6.
    Eisenach KD, Cave MD, Bates JH, Crawford JT: Polymerase chain reaction amplification of a repetitive DNA sequence specific forMycobacterium tuberculosis. Journal of Infectious Diseases 1990, 161: 977–981.PubMedGoogle Scholar
  7. 7.
    De Wit D, Steyn L, Shoemaker S, Sogin M: Direct detection ofMycobacterium tuberculosis in clinical specimens by DNA amplification. Journal of Clinical Microbiology 1990, 28: 2437–2441.PubMedGoogle Scholar
  8. 8.
    Hermans PW, Schuitema AR, Van Soolingem D, Versteynen CP, Bik EM, Thole JE, Kolk AH, van Embden JD: Specific detection ofMycobacterium tuberculosis complex strains by polymerase chain reaction. Journal of Clinical Microbiology 1990, 28: 1204–1213.PubMedGoogle Scholar
  9. 9.
    Pierre D, Lecossier D, Boussougant Y, Bocart D, Joly V, Yeni P, Hance AJ: Use of a reamplification protocol improves sensitivity of detection ofMycobacterium tuberculosis in clinical samples by amplification of DNA. Journal of Clinical Microbiology 1991, 29: 712–717.PubMedGoogle Scholar
  10. 10.
    Andersen AB, Thybo S, Godfrey-Faussett P, Stoker NG: Polymerase chain reaction for detection ofMycobacterium tuberculosis in sputum. European Journal of Clinical Microbiology and Infectious Diseases 1993, 12: 922–927.CrossRefGoogle Scholar
  11. 11.
    Noordhoek GT, Kolk AH, Bjune G, Catty D, Dale JW, Fine PEM, Godfrey-Faussett P, Cho SN, Shinnick T, Svenson SB, Wilson S, van Embden JDA: Sensitivity and specificity of PCR for detection ofMycobacterium tuberculosis: a blind comparison study among seven laboratories. Journal of Clinical Microbiology 1994, 32: 277–284.PubMedGoogle Scholar
  12. 12.
    Kubica GP, Dye E, Cohn ML, Middlebrook G: Sputum digestion and decontamination with N-acetyl-L-cystein sodiumhydroxide for culture of mycobacteria. American Review of Respiratory Disease 1963, 87: 775–779.PubMedGoogle Scholar
  13. 13.
    Silcox VA: Identification of mycobacteria. In: Isenberg HD (ed): Clinical microbiology procedures handbook. American Society for Microbiology, Washington DC, 1992, p. 3.11.1–3.11.11.Google Scholar
  14. 14.
    Abe C, Hirano Kg, Wada M, Kazumi Y, Takahashi M, Fukasawa Y, Yoshimura T, Miyagi C, Goto S: Detection ofMycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test. Journal of Clinical Microbiology 1993, 31: 3270–3274.Google Scholar
  15. 15.
    Jonas V, Alden MJ, Curry JI, Kamisango K, Knott CA, Lankford R, Wolfe JM, Moore DF: Detection and identification ofMycobacterium tuberculosis directly from sputum sediments by amplification of rRNA. Journal of Clinical Microbiology 1993, 31: 2410–2416.Google Scholar
  16. 16.
    Pfyffer GE, Kissling P, Wirth R, Weber R: Direct detection ofMycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system. Journal of Clinical Microbiology 1994, 32: 918–923.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1994

Authors and Affiliations

  • S. Ehlers
    • 1
  • M. Pirmann
    • 1
  • W. Zaki
    • 1
  • H. Hahn
    • 1
  1. 1.Institut für Medizinische Mikrobiologie und InfektionsimmunologieFreie Universität BerlinBerlinGermany

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