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Journal of Molecular Evolution

, Volume 24, Issue 3, pp 236–251 | Cite as

The secondary structure of human 28S rRNA: The structure and evolution of a mosaic rRNA gene

  • Jerome L. Gorski
  • Iris L. Gonzalez
  • Roy D. Schmickel
Article

Summary

We have determined the secondary structure of the human 28S rRNA molecule based on comparative analysis of available eukaryotic cytoplasmic and prokaryotic large-rRNA gene sequences. Examination of large-rRNA sequences of both distantly and closely related species has enabled us to derive a structure that accounts both for highly conserved sequence tracts and for previously unanalyzed variable-sequence tracts that account for the evolutionary differences in size among the large rRNAs.

Human 28S rRNA is composed of two different types of sequence tracts: conserved and variable. They differ in composition, degree of conservation, and evolution. The conserved regions demonstrate a striking constancy of size and sequence. We have confirmed that the conserved regions of large-rRNA molecules are capable of forming structures that are superimposable on one another. The variable regions contain the sequences responsible for the 83% increase in size of the human large-rRNA molecule over that ofEscherichia coli. Their locations in the gene are maintained during evolution. They are G+C rich and largely nonhomologous, contain simple repetitive sequences, appear to evolve by frequent recombinational events, and are capable of forming large, stable hairpins.

The secondary-structure model presented here is in close agreement with existing prokaryotic 23S rRNA secondary-structure models. The introduction of this model helps resolve differences between previously proposed prokaryotic and eukaryotic large-rRNA secondary-structure models.

Key words

28S rRNA RNA secondary structure Evolution Ribosome Translation 

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Copyright information

© Springer-Verlag New York Inc. 1987

Authors and Affiliations

  • Jerome L. Gorski
    • 1
  • Iris L. Gonzalez
    • 1
  • Roy D. Schmickel
    • 1
  1. 1.Department of Human GeneticsUniversity of PennsylvaniaPhiladelphiaUSA

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