Digestive Diseases and Sciences

, Volume 41, Issue 12, pp 2477–2481 | Cite as

Antagonistic effects of sulfide and butyrate on proliferation of colonic mucosa

A potential role for these agents in the pathogenesis of ulcerative colitis
  • Stefan U. Christl
  • Hans-Dieter Eisner
  • Gerda Dusel
  • Heinrich Kasper
  • Wolfgang Scheppach
Intestinal Disorders, Inflammatory Bowel Disease, And Irritable Bowel Syndrome

Abstract

It has been shown that feces of patients with ulcerative colitis uniformly contain sulfate reducing bacteria. Sulfide produced by these bacteria interferes with butyrate-dependent energy metabolism of cultured colonocytes and may be involved in the pathogenesis of ulcerative colitis. Mucosal biopsies from the sigmoid rectum of 10 patients (no caner, polyps, inflammatory bowel disease) were incubated with either NaCl, sodium hydrogen sulfide (1 mmol/L), a combination of both sodium hydrogen sulfide and butyrate (10 mmol/L), or butyrate. Mucosal proliferation was assessed by bromodeoxyuridine labeling of cells in S-phase. Compared to NaCl, sulfide increased the labeling of the entire crypt significantly, by 19% (p < 0.05). This effect was due to an expansion of the proliferative zone to the upper crypt (compartments 3–5), where the increase in proliferation was 54%. Sulfide-induced hyperproliferation was reversed when samples were coincubated with sulfide and butyrate. The study shows that sodium hydrogen sulfide induces mucosal hyperproliferation. Our data support a possible role of sulfide in the pathogenesis of UC and confirm the role of butyrate in the regulation of colonic proliferation and in the treatment of UC.

Key words

ulcerative colitis sulfide sulfate reduction butyrate proliferation 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Stefan U. Christl
    • 1
  • Hans-Dieter Eisner
    • 1
  • Gerda Dusel
    • 1
  • Heinrich Kasper
    • 1
  • Wolfgang Scheppach
    • 1
  1. 1.Department of MedicineUniversity of WürzburgWürzburgGermany

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