The effects of 2,4,5-trichlorophenoxyacetic acid and 2,3,7,8-tetrachlorodibenzo-P-dioxin on developing chicken embryos

  • Phillip M. Allred
  • John R. Strange

DOI: 10.1007/BF02097787

Cite this article as:
Allred, P.M. & Strange, J.R. Arch. Environ. Contam. Toxicol. (1977) 6: 483. doi:10.1007/BF02097787


Analytical grade 2,4,5-T and TCDD were dissolved in acetone and injected into the airspace of fertile chicken eggs. Toxicity studies suggest that the LD50 for 2,4,5-T is 133.1 mg of 2,4,5-T per kg of egg weight. The LD50 for TCDD was estimated to be 2.4 × 10−4 mg of TCDD per kg of egg weight. A partitioned chi-square analysis of the embryo viability data and the nonparallel dose response lines obtained from 2,4,5-T and TCDD suggest an independent mode of action for the two chemicals.

As a result of the injection of these two chemicals, increased liver weight to egg weight percentages occurred. Liver weight enlargement as a result of elevated nucleic acid content was ruled out based on the following observations: (1) both RNA and DNA levels per gram of liver tissue were depressed and (2) treatment group RNA to DNA ratios remained the same as controls.

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • Phillip M. Allred
    • 2
    • 1
  • John R. Strange
    • 3
    • 1
  1. 1.School of BiologyGeorgia Institute of TechnologyAtlantaUSA
  2. 2.Department of BiologyEmory UniversityAtlanta
  3. 3.Center for Chemical Hazard Assessment, Syracuse Research CorporationSyracuse

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