Effects of DDT on reproduction in multiple generations of beagle dogs

  • Alice Ottoboni
  • Glenn D. Bissell
  • Alfred C. Hexter


The effects of chronic oral exposure to 1, 5, and 10 mg of technical DDT/kg/day on: 1) age at puberty, length of gestation, fertility, success of pregnancy, litter size, and lactational ability of dams; 2) viability, survival to weaning, sex distribution and growth of pups; and 3) morbidity, mortality, organ/body weight ratios, gross and histologic abnormalities in all animals were studied through three generations of Beagle dogs. There were a total of 135 adult female and 63 adult male dogs in the project which produced 650 pups.

There were no statistically significant differences among control and DDT-treated dogs in any of the reproductive variables, with the exception of age at puberty of the females. DDT-treated females had their first estrous cycles 2 to 3 months earlier (P<.001) than the control dogs. Selected DDT-treated females, held for a second breeding period, had normal anestrous periods between their first and second estrous cycles. There was no effect of DDT on survival, growth, and sex distribution of pups, nor was there any influence on morbidity, mortality, gross or histologic findings in any of the dogs. All organ/body weight ratios were normal, with the possible exception of an increase in liver/body weight ratio in some DDT-treated animals.


Weight Ratio Adult Female Multiple Generation Histologic Finding Litter Size 
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  1. Anderson, A. C.: Puppy production to the weaning age. J. A. V. M. A.130 151 (1957).Google Scholar
  2. Anderson, A. C.: Reproduction. In: The Beagle As an Experimental Dog. Anderson, A. C. (ed), pgs. 31–39. Ames, Iowa: The Iowa State University Press (1970a).Google Scholar
  3. Anderson, A. C.: General pathology. In: The Beagle As an Experimental Dog. Anderson, A. C. (ed), pp. 520–546. Ames, Iowa: The Iowa State University Press (1970b).Google Scholar
  4. Anderson, A. C., and M. Goldman: Growth and development. In: The Beagle As an Experimental Dog. Anderson, A. C. (ed), pp. 43–105. Ames, Iowa: The Iowa State University Press (1970c).Google Scholar
  5. Anderson, A. C., and E. Wooten: The estrous cycle of the dog. In: Reproduction in Domestic Animals. Cole, H. H. and P. T. Cupps (eds), vol. 1, p. 359. New York: Academic Press (1959).Google Scholar
  6. Balazs, T.: Effects of DDD and DDT on the production and metabolism of adrenocortical steroids in guinea pigs and dogs. Am. J. Vet. Res.30 1535 (1969).PubMedGoogle Scholar
  7. Bartholomew, D. J.: Ordered tests in the analysis of variance. Biometrika48 325 (1961).Google Scholar
  8. Bernard, R. F., and R. A. Gaertner: Some effects of DDT on reproduction in mice. J. Mammal.45 272 (1964).Google Scholar
  9. Bissell, G. D., and A Ottoboni: The conduct of a multigeneration toxicity study in dogs. Lab Animal2 20 (1973).Google Scholar
  10. Cecil, H. C., J. Bitman, and S. J. Harris: Estrogenicity ofo,p-DDT in rats. J. Agr. Food Chem.19 61 (1971).Google Scholar
  11. Conley, B. E.: Pharmacologic and toxicologic aspects of DDT (chlorophenothane U.S.P.) J. Am. Med. Assoc.145 728 (1951).PubMedGoogle Scholar
  12. Conover, W. J.: Practical Nonparametric Statistics. pgs. 203–292. New York: John Wiley and Sons, Inc. (1971).Google Scholar
  13. Copeland, M. F., and M. F. Cranmer: Effects ofo,p-DDT on the adrenal gland and hepatic microsomal enzyme system in the Beagle dog. Toxicol. Appl. Pharmacol.27 1 (1974).PubMedGoogle Scholar
  14. Cueto, C., J. H. U. Brown, and A. P. Richardson, Jr.: Biological studies on an adrenocorticolytic agent and isolation of the active components. Endrocrinology62 334 (1958).Google Scholar
  15. Deichmann, W. B., W. E. MacDonald, A. G. Beasley, and D. Cubit: Subnormal reproduction in Beagle dogs induced by DDT and aldrin. Ind. Med. Surg.4 10 (1971).Google Scholar
  16. DeLong, R. L., W. G. Gilmartin, and J. G. Simpson: Premature births in California sea lions: Association with high organochlorine pollutant residue levels. Science181 1168 (1973).PubMedGoogle Scholar
  17. Duby, R. T., H. F. Travis, and C. E. Terrill: Uterotrophic activity of DDT in rats and mink and its influence on reproduction in the rat. Toxicol. Appl. Pharmacol.18 348 (1971).PubMedGoogle Scholar
  18. Fligner, M. A., and T. J. Killeen: Distribution-free two-sample tests for scale. J. Am. Statist. Assn.71 210 (1976).Google Scholar
  19. Hart, M. M., R. H. Adamson, and S. Fabro: Prematurity and intrauterine growth retardation induced by DDT in the rabbit. Arch. Int. Pharmacodyn.192 286 (1971).PubMedGoogle Scholar
  20. Heinrichs, W. L., R. J. Gellert, J. L. Bakke, and N. L. Lawrence: DDT administered to neonatal rats induces persistent estrus syndrome. Science173 642 (1971).PubMedGoogle Scholar
  21. Jefferies, D. J., and M. C. French: Avian thyroid: Effect ofp,p′-DDT on size and activity. Science166 1278 (1969).PubMedGoogle Scholar
  22. Jonckheere, A. R.: A distribution-free k-sample test against ordered alternatives. Biometrika41 133 (1954).Google Scholar
  23. Jukes, T. H.: DDT and tumors in experimental animals. Int. J. Environ. Stud.1 43 (1970).Google Scholar
  24. McGrath, J. P., McGrath, V.M.D.: Neurologic Examination of the Dog. pgs. 56–62. Philadelphia: Lee and Febiger (1956).Google Scholar
  25. McKelvie, D. H., and F. T. Shultz: Methods of observing and recording data in long-term studies on Beagles. Lab Animal Care14 118 (1964).Google Scholar
  26. Nichols, J., and G. Hennigar: Studies on DDD. Exp. Med. Surg.15 310 (1957).PubMedGoogle Scholar
  27. Ottoboni, A.: Effect of DDT on reproduction in the rat. Toxicol. Appl. Pharmacol.14 74 (1969).PubMedGoogle Scholar
  28. Ottoboni, A.: Effect of DDT on the reproductive life-span in the female rat. Toxicol. Appl. Pharmacol.22 497 (1972).PubMedGoogle Scholar
  29. Redman, H. C., A. J. Wilson, S. W. Bielfelt, and R. O. McClellan: Beagle dog production experience at the Fission Product Inhalation Program (1961–1968). Lab. Animal Care20 69 (1970).Google Scholar
  30. Roberts, S. J.: Veterinary Obstetrics and Genital Diseases. p. 103. Ann Arbor, Mich.: Edwards Bros., Inc. (1956).Google Scholar
  31. Tarján, R., and T. Kemény: Multigeneration studies on DDT in mice. Fd. Cosmet. Toxicol.7 215 (1969).Google Scholar
  32. Terpstra, T. J.: The asymptotic normality and consistency of Kendall's test against trend, when ties are present in one ranking. Indagationes Mathematicae14 327 (1952).Google Scholar
  33. Terpstra, T. J.: The exact probability distribution of the T statistic for testing against trend and its normal approximation. Indagationes Mathematicae15 433 (1953).Google Scholar
  34. Ware, G. W., and E. E. Good: Effect of insecticides on reproduction in the laboratory mouse. Toxicol. Appl. Pharmacol.10 54 (1967).PubMedGoogle Scholar
  35. Weil, C. S., and C. P. Carpenter: Abnormal values in control groups during repeated-dose toxicologic studies. Toxicol. Appl. Pharmacol.14 335 (1969).PubMedGoogle Scholar
  36. Welch, R. M., W. Levin, R. Kuntzman, M. Jacobson, and A. H. Conney: Effect of halogenated insecticides on the mechanism and uterotrophic action of estrogens in rats and mice. Toxicol. Appl. Pharmacol.19 234 (1971).PubMedGoogle Scholar
  37. Wrenn, T. R., J. R. Wood, G. F. Fries, and J. Bitman: Tests of estrogenicity in rats fed low levels ofo,p′-DDT. Bull. Environ. Contam. Toxicol.5 61 (1970).Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1977

Authors and Affiliations

  • Alice Ottoboni
    • 1
  • Glenn D. Bissell
    • 1
  • Alfred C. Hexter
    • 2
  1. 1.California Department of Health
  2. 2.School of Public HealthUniversity of CaliforniaBerkeley

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