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Differing proliferative responses in proximal and distal colons of growing rats fed food eaten by adenoma patients

  • Gastrointestinal Oncology
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Abstract

Animal dietary studies related to human colorectal carcinogenesis are usually based on AIN-76A diet, which is dissimilar to human food in source, preparation, and content. Our aim was to examine colonic epithelial proliferation in rats fed a diet based on the mean daily food intake of adenoma patients. Foods were prepared as reported by the adenoma patients and dehydrated; 64 Sprague-Dawley rats were fed either “human adenoma” or AIN-76A diet and every eight weeks, eight from each group were sacrificed. Both groups gained weight equally, had no colonic histological changes, but during the study showed progressive lengthening of colonic crypts (P<0.01) and decreased proliferation (P<0.05) in distal colons. Compared to controls, rats fed human adenoma diet had significantly longer crypts (P<0.01) and more labeled cells (P<0.05) at 32 weeks; overall they had increased proliferation (P<0.01), most significantly in the distal colon. Thus, food eaten by adenoma patients induced hyperproliferative changes in the rat colon during growth and maturity, especially the distal colon, as found in humans at risk for neoplasia.

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Authors and Affiliations

  1. From the Gastroenterology Department, Tel Aviv Medical Center and Tel Aviv University, Israel

    Vivian Liberman MSc, Abraham Nyska DVM, Hanoch Kashtan MD, Gershom Zajicek MD, Flora Lubin MSc &  Paul Rozen MB, BS

  2. Department of Pathology, Kimron Veterinary Institute, Bet Degen

    Vivian Liberman MSc, Abraham Nyska DVM, Hanoch Kashtan MD, Gershom Zajicek MD, Flora Lubin MSc &  Paul Rozen MB, BS

  3. H. Humphrey Center for Experimental Medicine and Cancer Research and Hebrew University, Jerusalem

    Vivian Liberman MSc, Abraham Nyska DVM, Hanoch Kashtan MD, Gershom Zajicek MD, Flora Lubin MSc &  Paul Rozen MB, BS

Authors
  1. Vivian Liberman MSc
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  2. Abraham Nyska DVM
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  3. Hanoch Kashtan MD
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  4. Gershom Zajicek MD
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  5. Flora Lubin MSc
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  6. Paul Rozen MB, BS
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Additional information

This paper represents part of the MSc thesis of Vivian Liberman for the Hebrew University. Supported in part by the Tel Aviv University H. Schauder Fund for Experimental Surgery (H. Kashtan).

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Liberman, V., Nyska, A., Kashtan, H. et al. Differing proliferative responses in proximal and distal colons of growing rats fed food eaten by adenoma patients. Digest Dis Sci 41, 1057–1064 (1996). https://doi.org/10.1007/BF02088219

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  • DOI: https://doi.org/10.1007/BF02088219

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