Digestive Diseases and Sciences

, Volume 39, Issue 9, pp 2038–2042

Prognostic value of cytosolic estrogen receptors in human colorectal carcinoma and surrounding mucosa

Preliminary results
  • A. Di Leo
  • C. Messa
  • F. Russo
  • G. Misciagna
  • V. Guerra
  • R. Taveri
  • S. Leo
Gastrointestinal Oncology

Abstract

Estrogen receptors have been found in normal and neoplastic gastrointestinal mucosa. The aim of our study was to verify whether the content of cytosolic estrogen receptor in normal and neoplastic tissue has a prognostic value in patients with colorectal adenocarcinoma. Eighty consecutive patients entered the study, and their follow-up was complete because non were lost to follow-up. Estrogen receptors were evaluated by an enzymatic immunoassay. Fifty-four percent of neoplastic samples and 84% of samples from surrounding mucosa showed an estrogen receptor content higher than 1.0 fmol/mg of cytosolic proteins (cut point for positive/negative hormone receptor status). Estrogen receptor levels were lower in neoplastic tissue than surrounding mucosa (1.2±1.05 fmol/mg protein vs 2.07±1.36 fmol/mg protein, respectively,t testP=0.001). The survival of patients with estrogen receptor expression in uninvolved surrounding mucosa was longer than that of patients without estrogen receptor in the same type of mucosa (log rank test,P<0.01). In the neoplastic tissue, receptor status had no prognostic value (log rank,P=0.8). After taking into account the most important potential confounders by using the Cox proportional hazard model, the estrogen receptor status in normal mucosa samples maintained an independent prognostic value. These results support an association between the estrogen receptor status in normal mucosa and survival of patients with colorectal adenocarcinoma.

Key words

estrogen receptors colorectal adenocarcinoma prognostic value 

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References

  1. 1.
    Utsumi Y, Nakamura T, Nagasue N, Kubota H, Morikawa S: Role of estrogen receptors in the growth of human esophageal carcinoma. Cancer 64:88–93, 1989PubMedGoogle Scholar
  2. 2.
    Tokunaka A, Nishi K, Matsukura R, Tanaka N, Onda M, Shirota A, Asano G, Hayashi K: Estrogen and progesterone receptors in gastric cancer. Cancer 57:1376–1379, 1986PubMedGoogle Scholar
  3. 3.
    Yamamoto N, Nakajo S, Tahara E: Immunohistochemical analysis of estrogen receptors in human gallbladder. Acta Pathol Jpn 40:14–21, 1990PubMedGoogle Scholar
  4. 4.
    Greenway B, Iqbal MJ, Johnson PJ, Williams R: Oestrogen receptor protein in malignant fetal pancreas. Br Med J 283:751–753, 1989Google Scholar
  5. 5.
    McClendon JE, Appleby D, Claudon DB, Donegan WL, De Cosse JJ: Colonic neoplasms, tissue estrogen receptor and carcinoembryonic antigen. Arch Surg 112:140–141, 1977Google Scholar
  6. 6.
    Alford TC, Do HM, Geelhead GW: Steroid hormone receptor in human colon cancer. Cancer 43:980–984, 1979PubMedGoogle Scholar
  7. 7.
    Sica V, Nola E, Contieri E: Estradiol and progesterone receptors in malignant gastrointestinal tumors. Cancer Res 44:4670–4674, 1984PubMedGoogle Scholar
  8. 8.
    Wobbes T, Beex LV, Koenders AM: Estrogen and progestinic receptors in colonic cancer. Dis Colon Rectum 27:51–52, 1984Google Scholar
  9. 9.
    Francavilla A, Di Leo A, Polimeno L, Conte D, Barone M, Fanizza G, Chiumarulo C, Rizzo G, Rubino M: Nuclear and cytosolic estrogen receptors in human colon carcinoma and in surrounding noncancerous colonic tissue. Gastroenterology 93:1301–1306, 1987PubMedGoogle Scholar
  10. 10.
    Takeda H, Yamakawa M, Takahashi T, Imai Y, Ishikawa M: An immunohistochemical study with an estrogen receptor-related protein (ER-D5) in human colorectal cancer. Cancer 69:907–912, 1992PubMedGoogle Scholar
  11. 11.
    Di Leo A, Linsalata M, Cavallini A, Messa C, Russo F: Sex steroid hormone receptors, epidermal growth factor receptor and polyamines in human colorectal cancer. Dis Colon Rectum 35:305–309, 1992PubMedGoogle Scholar
  12. 12.
    Russo F, Linsalata M, Messa C, Cavallini A, Notarnicola M, Guerra V, Lantone G, Lacatena M, Di Leo A: Polyamines and estrogen receptor concentrations in human colorectal carcinomas. Ital J Gastroenterol 24:8–12, 1992PubMedGoogle Scholar
  13. 13.
    Mc Guire WL, Carbone PP, Vollner EP: Estrogen Receptors in Human Breast Cancer. New York, Raven Press, 1975, pp 37–56Google Scholar
  14. 14.
    Soutter WP, Leake RE: Steroid hormone receptors in gynaecological cancers.In Recent Advances in Obstetrics and Gynaecology, Vol. 15. J Bonner (ed). Edinburgh, Churchill Livingstone, 1987, pp 175–194Google Scholar
  15. 15.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RS: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–267, 1951PubMedGoogle Scholar
  16. 16.
    Coffer AI, Lewis KM, Bockas AJ: Monoclonal antibodies against a component related to soluble antigen receptor. Cancer Res 45:3686–3693, 1985PubMedGoogle Scholar
  17. 17.
    Christensen E: Multivariate survival analysis using Cox's regression model. Hepatology 7:1346–1358, 1987PubMedGoogle Scholar
  18. 18.
    Dora J, Andre J, Gautier R, Burtin P: Abnormal pattern of mucus-associated M1 antigens in histologically normal mucosa adjacent to colonic adenocarcinomas. Cancer Res 44:4040–4045, 1984PubMedGoogle Scholar
  19. 19.
    Tayler R, Cumberland VH, Piper DW: Lactate dehydrogenase isoenzyme pattern in uninvolved mucosa of patients with colorectal carcinoma. Gut 18:45–47, 1977PubMedGoogle Scholar
  20. 20.
    Filipe MI, Branfoot AC: Abnormal pattern of mucus secretion in apparently normal mucosa of large intestine with carcinoma. Cancer 34:282–290, 1974PubMedGoogle Scholar
  21. 21.
    Langvad E: Lactate dehydrogenase isoenzyme pattern in the tumor bearing colon. Int J Cancer 3:17–29, 1968PubMedGoogle Scholar
  22. 22.
    La Muraglia GM, Lacaine F, Malt RA: High ornithine decarboxylase activity and polyamine levels in human colorectal neoplasia. Ann Surg 204:89–93, 1986PubMedGoogle Scholar
  23. 23.
    Coffey RJ, Shipley GD, Moses HL: Production of transforming growth factors by human colon cancer lines. Cancer Res 46:1164–1169, 1986PubMedGoogle Scholar
  24. 24.
    Goustin AS, Leof EB, Shipley GD, Moses HL: Growth factors and cancer. Cancer Res 46:1015–1029, 1986PubMedGoogle Scholar
  25. 25.
    Sporn MB, Roberts AB: Introduction: autocrine, paracrine, and endocrine mechanism and growth control. Cancer Surv 4:627–632, 1985Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • A. Di Leo
    • 1
  • C. Messa
    • 1
  • F. Russo
    • 1
  • G. Misciagna
    • 1
  • V. Guerra
    • 1
  • R. Taveri
    • 1
  • S. Leo
    • 1
  1. 1.From the Laboratorio di Biochimica; Laboratorio di Epidemiologia e Biostatistica; Unità OncologicaI.R.C.C.S., “S. de Bellis,”Castellana G. (BA)Italy

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