Digestive Diseases and Sciences

, Volume 39, Issue 9, pp 1872–1876 | Cite as

Nerve-mediated nitric oxide production by opossum lower esophageal sphincter

  • J. Murray
  • J. N. Bates
  • J. L. Conklin
Esophageal, Gastric, And Duodenal Disorders

Abstract

Antagonists of nitric oxide synthesis inhibit nerve-induced hyperpolarization and relaxation of muscle from the opossum lower esophageal sphincter. These studies test the hypothesis that nitric oxide is released during stimulation of intrinsic esophageal nerves. The intrinsic nerves were stimulated with an electrical field (10-sec trains of 1-msec, 30-V pulses delivered at 10 Hz). Nitric oxide production was measured with a DASIBI model 2108 Chemiluminescence NO Analyzer.NG-Nitro-l-arginine, an inhibitor of NO synthase, antagonized nerve-induced relaxation the lower esophageal sphincter. Nerve stimulation increased NO production from 0.50±0.04 nmol/min/100 mg tissue to 0.87±0.07 nmol/min/100 mg tissue (P<0.01).NG-nitro-l-arginine inhibited both basal (0.030±0.09 nmol/min/100 mg tissue,P<0.05 vs baseline) and stimulated (0.38±0.10 nmol/min/100 mg tissue,P<0.01 vs stimulated). These studies support the hypothesis that nerve stimulation releases nitric oxide from the lower esophageal sphincter.

Key words

enteric nervous system nonadrenergic noncholinergic nerves esophagus gastrointestinal motility peristalsis 

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • J. Murray
    • 1
  • J. N. Bates
    • 1
  • J. L. Conklin
    • 1
  1. 1.From the Departments of Internal Medicine and AnesthesiologyUniversity of Iowa College of Medicine and Department of Veterans Affairs Medical CenterIowa City

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