Agents and Actions

, Volume 20, Issue 3–4, pp 277–280 | Cite as

Diamine oxidase (DAO) activity and intestinal mucosa integrity: influence of suture techniques

  • R. Mennigen
  • J. Kusche
  • L. Leisten
  • K. Erpenbach
Histamine Receptors and Metabolism


After various kinds of intestinal mucosal injuries, whether by disease or by experiment, the diamine oxidase activity is reduced. Therefore, we studied the effect of surgical manipulations on the intestinal mucosa and diamine oxidase activity. The reaction of the gut on the insertion of sutures was a transient increase of the enzymic activity followed by reduction as soon as the mucosa started to gain weight. After a standardized pressure injury only a reduction of the diamine oxidase activity together with an enhancement of the mass of the intestinal wall was found. A hypothesis of a feed-back regulation of the diamine oxidase activity connected with mucosal proliferation is proposed.


Enzymic Activity Diamine Oxidase Activity Intestinal Mucosa Transient Increase 
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  1. [1]
    G. D. Luk, T. M. Bayless and S. B. Baylin,Diamine oxidase (histaminase) a circulating marker for rat intestinal mucosal maturation and integrity. J clin. Invest.66, 66–70 (1980).PubMedGoogle Scholar
  2. [2]
    J. Kusche, W. Lorenz and R. Hesterberg,The relevance of the diamine-oxidase-histaminase system for shock development following intestinal ischemia. In:Animal models for intestinal disease. (Ed. L. J. Pfeiffer) pp. 255–280. CRC press, Boca Raton USA 1985.Google Scholar
  3. [3]
    R. Mennigen, M. Günther, N. Bönninghoff, C.-D. Stahlknecht, I. Kubitzka and J. Kusche,Diamine oxidase as a marker of intestinal integrity in acute appendicitis. Agents and Actions18, 38–40 (1986)PubMedGoogle Scholar
  4. [4]
    R. Hesterberg, J. Kusche, C.-D. Stahlknecht and K.-D. Feußner,The start of a programme for measuring diamine oxidase activity in biopsy specimens of human rectal mucosa. Agents and Actions11, 33–37 (1981).PubMedGoogle Scholar
  5. [5]
    J. Kusche, R. Mennigen and J. R. Izbicki,Diamine oxidase, the regulator of mucosa proliferation? In:Recent progress in polyamine research (Eds. L. Selmeci, M. E. Brosnan, N. Seiler) pp. 329–338. Académiai Kiadò Budapest and VNU, The Netherlands 1985.Google Scholar
  6. [6]
    J. Kusche and W. Lorenz, Diamine oxidase. In:Methods of enzymatic analysis. (Ed. H. U. Bergmeyer) 3rd ed. Vol. 3, pp. 237–250. Verlag Chemie, Weinheim, FRG 1983.Google Scholar
  7. [7]
    O. H. Lowry, N. J. Rosenbrough, A. L. Farr and R. J. Randall,Protein measurements with folin phenol reagent. J. biol. Chem.193, 265–275 (1951).PubMedGoogle Scholar
  8. [8]
    K. M. Shakir, S. Margolis and S. B. Baylin,Localization of histaminase (diamine oxidase) in rat small intestinal mucosa: site of release by heparin. Biochem. Pharmacol.26, 2343–2347 (1977).PubMedGoogle Scholar
  9. [9]
    J. Kusche, H. Richter, Ch. Specht, J. Schmidt and W. Lorenz,Alteration of diamine oxidase activity during intestinal ischemia in dogs. Proc. of the 6th Int. Congr. of Pharmacology, Helsinki, No. 544, p. 236 (1975).Google Scholar

Copyright information

© Birkhäuser Verlag 1987

Authors and Affiliations

  • R. Mennigen
    • 2
  • J. Kusche
    • 1
  • L. Leisten
    • 1
  • K. Erpenbach
    • 1
  1. 1.Biochemical and Experimental Division of the University of CologneKöln 91FRG
  2. 2.IInd. Department of Surgery of the University of CologneKöln 91FRG

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