Journal of Assisted Reproduction and Genetics

, Volume 13, Issue 2, pp 177–181 | Cite as

Expression of homeobox-containing genes in human preimplantation development and in embryos with chromosomal aneuploidies

  • Anver Kuliev
  • Valery Kukharenko
  • Gennady Morozov
  • Michael Freidine
  • Svetlana Rechitsky
  • Oleg Verlinsky
  • Victor Ivakhnenko
  • Victor Gindilis
  • Charles Strom
  • Yury Verlinsky
Papers From The International Working Group On Preimplantation Genetics

Abstract

Purpose: The purpose of the study was to investigate homeobox gene expression in human oocytes and preembryos and in postimplantation embryos with impaired embryonic development determined by chromosomal abnormalities.

Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) with intron spanning primer sets for Homeobox gene sequences was used.

Results: The homeobox genes HoxA4, HoxA7, HoxB4, and HoxB5 were present in human oocytes and cleaving normal and triploid embryos. The expression pattern was different between chromosomally abnormal and normal first-trimester embryos. Of four homeobox transcripts (HoxA7, HoxB4, HoxB5, and HoxC6) that are expressed in diploid embryos, only HoxA7, HoxB4, and HoxC6 were present in a trisomy 7 embryo, and only HoxB4 and HoxB5 in triploid embryos and an embryo with trisomy 9. Cloning experiments revealed differences in the number of homeobox clones obtained from trisomy 7 and control embryos.

Conclusions: The transcipts of homeobox genes, HoxA4, HoxA7, HoxB4, and HoxB5, were present in oocytes and cleaving embryos. The pattern of expression of homeobox genes in cultured fibroblasts derived from spontaneously aborted embryos with aneuploidies was different from that in control diploid cells.

Key words

Homeobox preimplantation development aneuploidy triploidy spontaneously aborted embryos 

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Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • Anver Kuliev
    • 1
  • Valery Kukharenko
    • 1
    • 2
  • Gennady Morozov
    • 1
  • Michael Freidine
    • 1
  • Svetlana Rechitsky
    • 1
  • Oleg Verlinsky
    • 1
  • Victor Ivakhnenko
    • 1
  • Victor Gindilis
    • 1
  • Charles Strom
    • 1
  • Yury Verlinsky
    • 1
  1. 1.Reproductive Genetics InstituteChicago
  2. 2.National Research Center for Medical GeneticsRAMSMoscowRussia

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