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Growth characteristics ofChattonella antiqua

Part 2. Effects of nutrients on growth
  • Yasuo Nakamura
  • Makoto M. Watanabe
Article

Abstract

Nutrient requirements of a red tide flagellate,Chattonella antiqua, were investigated in a laboratory culture experiment. Growth ofC. antiqua was supported by nitrate and ammonium, and by urea to a limited extent, but not by glycine, alanine and glutamate. Orthophosphate served as a good phosphorus source but glycerophosphate did not. Fe3+ (1µM) fully promoted the flagellate's growth in the presence of 80µM of EDTA. The addition of Mn2+ (0–20µM), Zn2+ (0–10µM) and Co2+ (0–0.4µM) did not show any effect. Among three vitamins tested, only B12 (6 ng 1−1) served as a growth factor. Glucose, acetate and glycolate did not improve growth in the light nor did they support growth in darkness. The minimum cell quotas for nitrogen, phosphorus, iron and B12 were estimated to be 11 pmoles ce−1, 1.0, ∼0.09 and 1.1 fg cell−1, respectively.

Keywords

Phosphorus Glutamate Glycolate Glycine Alanine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson, D. M. and F. M. Morel (1978): Copper sensitivity ofGonyaulax tamarensis. Limnol. Oceanogr.,23, 283–295.Google Scholar
  2. Endo, T., H. Koyama and H. Imabayashi (1982): Phytoplankton in Harima-Nada in relation to oceanographic conditions, particularly nutrient and physiological properties of seawater. In: Fundamental Studies on the Effects of Marine Environment on the Outbreaks of Red Tides. The Ministry of Education, Science and Culture, Japan. B148-R14-8. p. 81–92 (in Japanese).Google Scholar
  3. Guillard, R. R. L. and D. Cassie (1963): Minimum cyanocobalamine requirements of some marine centric diatoms. Limnol. Oceanogr.,11, 410–413.Google Scholar
  4. Hata, Y. and T. Nishijima (1982): Distribution of aerobic heterotrophic bacteria and B group vitamins in Harima Nada. In: Fundamental Studies on the Effects of Marine Environment on the Outbreaks of Red Tides. The Ministry of Education, Science and Culture, Japan. B148-R14-8. p. 109–120 (in Japanese).Google Scholar
  5. Iwasaki, H. (1971): Studies on the red tide flagellates IV. OnEutreptiella sp. andExuviaella sp. appeared in Bingo-Nada, the Seto Inland Sea, in 1970. J. Oceanogr. Soc. Japan,27, 152–157 (in Japanese).Google Scholar
  6. Iwasaki, H. (1979): Physiological ecology of red tide flagellates. In: Biochemistry and Physiology of Protozoa. Vol. 1, ed. by M. Levandousky and S. H. Hutner, Academic Press, New York, p. 357–393.Google Scholar
  7. Iwasaki, H. and K. Sasada (1969): Studies on the red tide flagellates. II. OnHeterosigma inlandica appeared in Gokasho Bay. Bull. Jap. Soc. Sci. Fish.,35, 943–947 (in Japanese).Google Scholar
  8. Lehman, J.T., D.B. Botokin and G.E. Likens (1975): The assumption and rationales of a computor model of phytoplankton population dynamics. Limnol. Oceanogr.,20, 343–364.Google Scholar
  9. Matsunaga, K., K. Igarashi, I. Kudo and S. Fukase (1982): Osaka-wan Yodo-gawa kako ni okeru jukinzoku nodo. In: Proc. 1982 Spring Meet. Oceanogr. Soc. Japan. p. 144 (in Japanese).Google Scholar
  10. Nakamura, Y. and M.M. Watanabe (1983): Growth characteristics ofChattonella antiqua. Part 1. Effects of temperature, salinity, light intensity and pH on growth. J. Oceanogr. Soc. Japan,39, 89–93.Google Scholar
  11. Nakamura, Y., M. M. Watanabe and M. Watanabe (1982): The effect of various environmental factors on the growth yield of red tide algae. I.Chattonella antiqua. Res. Rep. Natl. Inst. Environ. Stud., No. 30, 53–70 (in Japanese).Google Scholar
  12. Okaichi, T., S. Montani and T. Ochi (1982): Marine environment studies on the outbreaks of red tide due toChattonella antiqua (Hada) Ono in the Harima-Nada. In: Fundamental Studies on the Effects of Marine Environment on the Outbreaks of Red Tides. The Ministry of Education, Science and Culture, Japan. B148-R14-8. p. 93–108.Google Scholar
  13. O'Kelley, J.C. (1974): Inorganic nutrients. In: Algal Physiology and Biochemistry, ed. by W. D. P. Stewart, Univ. California Press., Berkeley and Los Angels, p. 610–635.Google Scholar
  14. Ono, C. and T. Takano (1980)Chattonella antiqua (Hada) comb. nov., and its occurrence on the Japanese coast. Bull. Tokai Reg. Fish. Res. Lab.,102, 93–100.Google Scholar
  15. Provasoli, L., J. J. A. McLaughlin and M. R. Droop (1957): The development of artificial media for marine algae. Arch. Mikrobiol.,25, 392–428.Google Scholar
  16. Rhee, G-Y. and J. Gotham (1980): Optimum N:P ratios and coexistence of planktonic algae. J. Physiol.,16, 486–489.Google Scholar
  17. Strickland, J.D.H., O. Holm-Hansen, R.W. Eppley and R.J. Linn (1969): The use of deep tank in plankton ecology. I. Studies of the growth and combination of phytoplankton crops at low nutrient levels. Limnol. Oceanogr.,14, 23–34.Google Scholar

Copyright information

© the Oceanographical Society of Japan 1983

Authors and Affiliations

  • Yasuo Nakamura
    • 1
  • Makoto M. Watanabe
    • 1
  1. 1.Water and Soil Environment Divisionthe National Institute for Environmental StudiesIbarakiJapan

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