Growth characteristics ofChattonella antiqua

Part 2. Effects of nutrients on growth
  • Yasuo Nakamura
  • Makoto M. Watanabe


Nutrient requirements of a red tide flagellate,Chattonella antiqua, were investigated in a laboratory culture experiment. Growth ofC. antiqua was supported by nitrate and ammonium, and by urea to a limited extent, but not by glycine, alanine and glutamate. Orthophosphate served as a good phosphorus source but glycerophosphate did not. Fe3+ (1µM) fully promoted the flagellate's growth in the presence of 80µM of EDTA. The addition of Mn2+ (0–20µM), Zn2+ (0–10µM) and Co2+ (0–0.4µM) did not show any effect. Among three vitamins tested, only B12 (6 ng 1−1) served as a growth factor. Glucose, acetate and glycolate did not improve growth in the light nor did they support growth in darkness. The minimum cell quotas for nitrogen, phosphorus, iron and B12 were estimated to be 11 pmoles ce−1, 1.0, ∼0.09 and 1.1 fg cell−1, respectively.


Phosphorus Glutamate Glycolate Glycine Alanine 
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Copyright information

© the Oceanographical Society of Japan 1983

Authors and Affiliations

  • Yasuo Nakamura
    • 1
  • Makoto M. Watanabe
    • 1
  1. 1.Water and Soil Environment Divisionthe National Institute for Environmental StudiesIbarakiJapan

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