Optical and Quantum Electronics

, Volume 18, Issue 1, pp 81–84 | Cite as

Highly sensitive fluorescence microscope system for single biological cell using laser excitation and digital image processing and display

  • Shun-ichi Sato
  • Hiromasa Ito
  • Humio Inaba
  • Yoshio Taguchi
  • Morio Kasai
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Abstract

A novel type of laser-excited fluorescence microscope system is reported, which, for the first time, realizes excellent sensitivity and spatial resolution in biological and medical applications using an ultra-high sensitivity image camera and digital image processing technique for three-dimensional display of the detected fluorescence intensity across a single cell. Experimental studies are also presented on the fluorescence intensity distribution of Rhodamine 6G adsorbed in an OH gel as a simple cell model and also of a hematoporphyrin-derivative (HpD) taken up by a cultured esophagus cancer cell.

Keywords

Fluorescence Intensity Rhodamine Image Camera Digital Image Processing Image Processing Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Chapman and Hall Ltd. 1986

Authors and Affiliations

  • Shun-ichi Sato
    • 1
  • Hiromasa Ito
    • 1
  • Humio Inaba
    • 1
  • Yoshio Taguchi
    • 2
  • Morio Kasai
    • 2
  1. 1.Research Institute of Electrical CommunicationTohoku UniversitySendaiJapan
  2. 2.The 2nd Department of SurgeryTohoku University School of MedicineSendaiJapan

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