Journal of Assisted Reproduction and Genetics

, Volume 13, Issue 9, pp 733–738 | Cite as

Quality control in IVF with mouse bioassays: A four years' experience

  • M. van den Bergh
  • I. Baszó
  • J. Biramane
  • E. Bertrand
  • F. Devreker
  • Y. Englert
Animal Experimentation


Objective: We analyzed retrospectively the relevance of 4 years of quality control on homemade culture medium with the mouse IVF and zygote bioassay.

Design: In vitro or in vivo fertilized mouse oocytes were cultured in each batch of medium. Two-cell-stage and expanded blastocyst development was recorded for each batch of medium. Data on fertilization and embryo quality obtained in human in vitro fertilization were recorded for each batch. IVF treatment cycles for male infertility and cycles with sperm microinjection were excluded.

Results: Human oocyte fertilization dropped from 60 to 54%, respectively, from 57 to 41% in a significant way (P<0.05 resp. P<0.01) and the human mean embryo score decreased from 4.17±1.21 to 3.69±1.06 (P<0.05) when media were used with a low two-cell-stage development (≤75%) for the mouse zygote or mouse IVF bioassay. The pregnancy rate was not affected.

Conclusions: Media with high scores in mouse bioassays show higher fertilization rates and better embryo quality when used for human IVF.

Key words

mouse zygote bioassay quality control human in vitro fertilization fertilization rate embryo quality 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Critchlow J, Matson P, Newman M, Horne G, Troup S, Lieberman A: Quality control in an in-vitro fertilization laboratory: Use of human sperm survival studies. Hum Reprod 1989;4:545–549PubMedGoogle Scholar
  2. 2.
    Bavister B, Andrews J: A rapid sperm motility bioassay for quality control testing of water and culture media. J Vitro Fert Embryo Transfer 1988;5:67–74Google Scholar
  3. 3.
    Reed M, Petters R: An evaluation of hamster, rat, and mouse sperm cell motility in media formulated in media with water of different qualities. J Vitro Fert Embry Transfer 1991;8:41–47Google Scholar
  4. 4.
    Ackerman S, Swanson R, Stokes G, Veeck L: Culture of mouse preimplantation embryos as a quality control assay for human in vitro fertilization. Gamete Res 1984;9:145–152Google Scholar
  5. 5.
    Bavister B: A consistently successful procedure for in vitro fertilization of golden hamster eggs. Gamete Res 1989;23:139–158PubMedGoogle Scholar
  6. 6.
    Davidson A, Vermesh M, Lobo R, Paulson R: Mouse embryo culture as quality control for human in vitro fertilization: The one-cell versus the two-cell model. Fertil Steril 1988;49:516–521PubMedGoogle Scholar
  7. 7.
    George M, Doe B: The influence of handling procedures during mouse oocyte and embryo recovery on viability and subsequent development in vitro. J Vitro Fert Embryo Transfer 1989;6:69–72Google Scholar
  8. 8.
    Scott L, Sundaram S, Smith S: The relevance and use of mouse embryo bioassays for quality control in an assisted reproductive technology program. Fertil Steril 1993;60:559–568PubMedGoogle Scholar
  9. 9.
    Hogan B, Constantini F, Lacy E: Manipulating the Mouse Embryo: A Laboratory Manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, 1986Google Scholar
  10. 10.
    Englert Y, Van den Bergh M, Rodesch C, Vandervorst P, Berberoglugil P, Laruelle C, Biramane J, Gervy C, Schwers J: Nouveau programme de fécondation in vitro à l'hôpital Erasme: premiers résultats et aspects originaux. Rev Med Brux 1991;12:305–314PubMedGoogle Scholar
  11. 11.
    Puissant F, Van Rysselberge M, Barlow P, Deweze J, Leroy F: Embryo scoring as a prognostic tool in IVF treatment. Hum Reprod 1987;2:705–708PubMedGoogle Scholar
  12. 12.
    Gerrity M: Mouse embryo culture essay.In Wolf D, Bavister D, Gerrity M, Kopf G (eds), In Vitro Fertilization and Embryo Transfer: A Manual of Basic Techniques. New York: Plenum Press, 19xx, pp 57–76Google Scholar
  13. 13.
    Johnson D, Hodgen G: Syringe-associated toxicity of culture media on mouse ans monkey preembryos. J Vitro Fert Embryo Transfer 1991;8:198–201Google Scholar
  14. 14.
    Rinehart J, Bavister B, Gerrity M: Quality control in the in vitro fertilization laboratory: Comparison of bioassay systems for quality water. J Vitro Fert Embryo Transfer 1988;5:335–342Google Scholar
  15. 15.
    Fleming T, Prath H, Braude P: The use of mouse preimplantation embryos for quality control of culture reagents in human in vitro fertilization programs: Acautionary note. Fertil Steril 1987;47:858–860PubMedGoogle Scholar
  16. 16.
    Montora L, Subias E, Young P, Baccaro M, Swanson J, Sueldo C: Detection of endotoxin in human in vitro fertilization by the zona-free mouse embryo essay. Fertil Steril 1990;54:109–112PubMedGoogle Scholar
  17. 17.
    Fishel S, Jackson P, Webster J, Faratian B: Endotoxins in culture medium for human in vitro fertilization. Fertil Steril 1988;49:108–111PubMedGoogle Scholar
  18. 18.
    Snyman E, Van der Werve J: Endotoxin-polluted medium in a human in vitro fertilization program. Fertil Steril 1986;46:273–276PubMedGoogle Scholar
  19. 19.
    George M, Braude P, Johnson M, Sweetnam D: Quality control in the IVF laboratory: In vitro and in vivo development of mouse embryos is unaffected by the quality of water used in culture media. Hum Reprod 1989;4:826–831PubMedGoogle Scholar
  20. 20.
    Dankekar P, Glass R: Development of mouse embryos is affected by strain and culture medium. Gamete Res 1987;17:279–285PubMedGoogle Scholar
  21. 21.
    Jackson K, Kiessling A: Fertilization and cleavage of mouse oocytes exposed to the conditions of human oocyte retrieval. Fertil Steril 1989;51:675–681PubMedGoogle Scholar
  22. 22.
    Bavister B: Culture of preimplantation embryos: Facts and artefacts. Hum Reprod Update 1995;1:91–148PubMedGoogle Scholar
  23. 23.
    Endo A, Watanabe T: Effects of non-24-days on reproductive efficacy and embryonic development in mice. Gamete Res 1989;22:435–441PubMedGoogle Scholar
  24. 24.
    Dodson M, Minhas B, Curtis S, Palmer T, Robertson L: Spontaneaous zona reaction in the mouse as a limiting factor for the time in which an oocyte may be fertilized. J Vitro Fert Embryo Transfer 1989;6:101–106Google Scholar
  25. 25.
    Clarke R, Griffin P, Biggers J: Screening of maternal serum is not predictive of human embryo development or IVF outcome. J Assist Reprod Genet 1995;12:20–25PubMedGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1996

Authors and Affiliations

  • M. van den Bergh
    • 1
  • I. Baszó
    • 2
  • J. Biramane
    • 1
  • E. Bertrand
    • 1
  • F. Devreker
    • 1
  • Y. Englert
    • 1
  1. 1.Fertility Clinic, Hôpital ErasmeFrench-Speaking Free University of BrusselsBrusselsBelgium
  2. 2.Institut Supérieur De Mot-CouvreurBrusselsBelgium

Personalised recommendations