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Journal of Chemical Ecology

, Volume 20, Issue 8, pp 1907–1921 | Cite as

Surface disposition and stability of pest-interactive, trichome-exuded diterpenes and sucrose esters of tobacco

  • Y. Lin
  • G. J. Wagner
Article

Abstract

The precise physical location of trichome-exudate biochemicals on the plant surface is undoubtedly important in plant-pest interactions, perhaps particularly those involving fungal and bacterial pathogens that invade the plant through the epidermal layer. The chemical stability of exuded compounds is also important in this regard. Here we have studied these two aspects of trichome biology using the highly exuded tobacco line,Nicotiana tabacum, T.I. 1068. Particularly under high relative humidity growth conditions, sucrose esters (SE) were found to migrate from the exudate droplet around the gland down the trichome stalk to the epidermal cells below. Six days after labeling leaf midveins on plants grown in a high humidity environment, 29 and 71% of label found in SE were recovered with trichome glands and below gland regions, respectively. Corresponding disposition in the moderate humidity environment was 40 and 60%, respectively. Migration of less polar duvatrienediols (DVT) was less marked. Staining of SE with rhodamine B showed the occurrence of more extensive and physically different migration in the high humidity versus moderate humidity case. Both SE and DVT were stable between six and 18 days postlabeling, the period encompassing the time of maximum exudate formation through the beginning of tissue senescence. Our results suggest that even under conditions that avoid mechanical disturbance of tissue, SE and DVT are chemically stable, at least until senescence, and appear to migrate from the gland region to the epidermal surface, apparently according to their relative polarity.

Key words

Trichome trichome exudate surface deposition exudate stability surface chemicals sucrose esters duvatrienes terpenes pest resistance humidity tobacco 

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References

  1. Breccia, A., andBadiello, R. 1967. The role of general metabolites in the biosynthesis of natural products: 1. The terpene marrubiin.A. Naturforsch. 22b:44–49.Google Scholar
  2. Croteau, R. 1986. Catabolism of monoterpenes in essential oil plants, pp. 65–84,in B.M. Lawrence, B.D. Mookherjee, and B.J. Willis, (eds.). Flavors and Fragrances: A World Perspective. Proceedings of the 10th International Congress of Essential Oils, Fragrances and Flavors. Elsevier Science Publishers, Amsterdam.Google Scholar
  3. Cutler, H.G., Severeson, R.F., Cole, P.D., Sisson, V.A., Jackson, M.D., andStephenson, M.G. 1992. The biological activity of some mixed sucrose and glucose esters from exotic tobacco cultivars.PGRSA Q. 20:18–28.Google Scholar
  4. Dell, B., andMcComb, A.J. 1978. Plant resins—their formation, secretion and possible functions.Adv. Bot. Res. 6:227–316.Google Scholar
  5. Duffey, S.S. 1986. Plant glandular trichomes: Their partial role in defence against insects, pp. 151–172,in B.E. Juniper and T.R.E. Southwood (eds.). Insects and the Plant Surface. Edward Arnold, London.Google Scholar
  6. Fahn, A. 1988. Secretory tissues in vascular plants.New Physiol. 108:229–257.Google Scholar
  7. Guo, Z., Severson, R.F., andWagner, G.J. 1994. Biosynthesis of the diterpenecis-abienol in cell-free extracts of tobacco trichomes.Arch. Biochem. Biophys. 308:103–108.PubMedGoogle Scholar
  8. Kandra, L., andWagner, G.J. 1988. Studies of the site and mode of biosynthesis of tobacco trichome exudate components.Arch. Biochem. Biophys. 265:425–432.PubMedGoogle Scholar
  9. Keene, C.K., andWagner, G.J. 1985. Direct demonstration of duvatrienediol biosynthesis in glandular heads of tobacco trichomes.Plant Physiol. 79:1026–1032.Google Scholar
  10. Kelsey, R.G., Reynolds, G.W., andRodriguez, E. 1984. The chemistry of biologically active constituents secreted and stored in plant glandular trichomes, pp. 187–241,in E. Rodreguez, P.L. Healey, and I. Mehta (eds.). Biology and Chemistry of Plant Trichomes. Plenum Press, New York.Google Scholar
  11. Kennedy, B.S., Nielsen, M.T., Severson, R.F., Sisson, V.S., Stephenson, M.K., andJackson, D.M. 1992. Leaf surface chemicals fromNicotiana affecting germination ofPeronospora tabacina (ADAM) sporangia.J. Chem. Ecol. 18:1467–1479.Google Scholar
  12. McCaskill, D., Gershenson, J., andCroteau, R. 1992. Morphology and monoterpene biosynthetic capabilities of secretory cell clusters isolated from glandular trichomes of peppermint (Mentha piperita L.)Planta 187:445–454.Google Scholar
  13. Menetrez, M.L., Spurr, H.W., Jr., Danehower, D.A., andLawson, D.R. 1990. Influence of tobacco leaf surface chemicals on germination ofPeronospora tabacina (ADAM) sporangia.J. Chem. Ecol. 16:1565–1576.Google Scholar
  14. Nicholas, H.J. 1964. Biosynthesis and metabolism of [14C]sclareol.Biochim. Biophys. Acta 84:80–90.PubMedGoogle Scholar
  15. Severson, R.F., Johnson, A.W., andJackson, D.M. 1985. Cuticular constituents of tobacco: Factors affecting their production and their role in insect and disease resistance and smoke quality.Recent Adv. Tobacco Sci. 11:105–174.Google Scholar
  16. Severson, R.F., Jackson, D.M., Johnson, A.W., Sisson, V.A., andStephenson, M.G. 1991. Ovipositional behavior of tobacco budworm and tobacco hornworm: Effects of cuticular components fromNicotiana species, pp. 264–277,in P.A. Hedin (ed.). Naturally Occurring Pest Bioregulators. American Chemical Society Symposium Series 449. American Chemical Society, Washington, D.C.Google Scholar
  17. Tingey, W.M. 1991. Potato glandular trichomes: Defensive activity against insect attack, pp. 127–135,in P.A. Hedin (ed.). Naturally Occurring Past Bioregulators. American Chemical Society Symposium Series 449. American Chemical Society, Washington, D.C.Google Scholar
  18. Wagner, G.J. 1991. Secreting glandular trichomes: more than just hairs.Plant Physiol. 96:675–679.Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Y. Lin
    • 1
  • G. J. Wagner
    • 1
  1. 1.Plant Physiology/Biochemistry/Molecular Biology Program Department of AgronomyUniversity of KentuckyLexington

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