Decreased mucosal IgA levels in ileum of patients with chronic ulcerative colitis
Patients with chronic ulcerative colitis (CUC) are known to have decreased spontaneous IgA secretion by colonic mononuclear cells. The aim of this study was to determine whether a similar alteration exists in the apparently healthy ileum of patients with CUC. The concentration of IgA was measured in the supernatant from homogenized mucosal ileal biopsies using a sandwich-type ELISA. The concentration of IgA was significantly (P=0.025) decreased in the ileum of patients with CUC (N=24) in comparison to normal ileum (N=10). The number of mucosal IgA-containing mononuclear cells (MNC) was also determined using an avidin-biotin-immunoperoxidase technique on paraffin-embedded ileal sections. Although reduced, the number of positive cells and their distribution was not significantly different in the ileum of patients with CUC (N=20) when compared to normal ileum (N=10). We suggest that decreased mucosal IgA levels are a panintestinal condition in CUC and that this is a primary alteration rather than a secondary response to the inflammatory process. Considering the role of IgA, we propose that decreased mucosal IgA levels in CUC may predispose to the disease by a reduction of the immune-mediated exclusion mechanism and/or by an impairment of the down-regulation of the inflammatory response.
Key wordsimmunoglobulin A ulcerative colitis ileum
Unable to display preview. Download preview PDF.
- 2.MacDermott RP, Beale MG, Alley CD, Nash GS, Bertovich MJ, Bragdon MJ: Synthesis and secretion of IgA, IgM and IgG by peripheral blood mononuclear cells in human disease states, by isolated human intestinal mononuclear cells, and by human bone marrow mononuclear cells from ribs. Ann NY Acad Sci 83:498–509, 1983Google Scholar
- 6.Brandtzaeg P: Immunopathology of inflammatory bowel disease.In Inflammatory Bowel Disease. RP MacDermott, WF Stenson, (eds). New York, Elsevier, 1992, pp 102–136Google Scholar
- 12.Crama-Bohbouth G, Pena AS, Verspaget HW, Zon A, Biemont I, Weterman IT, Lamers CBHW: Immunological findings in whole and parotid saliva of patients with ulcerative colitis and healthy controls. Hepato-Gastroenterol 36:185–187, 1989Google Scholar
- 14.Castro GA: Gut immunophisiology: Regulatory pathways within a common mucosal immune system. News Physiol Sci 4:59–64, 1989Google Scholar
- 28.Walker WA, Wu M, Bloch KJ: Stimulation by immune complexes of mucus release from goblet cells of the rat small intestine. Science 97:370–371, 1977Google Scholar