Digestive Diseases and Sciences

, Volume 40, Issue 5, pp 949–959

Elevation of interleukin-6 in inflammatory bowel disease is macrophage- and epithelial cell-dependent

  • Kazuo Kusugami
  • Atsushi Fukatsu
  • Mitsune Tanimoto
  • Masataka Shinoda
  • Jun-Ichi Haruta
  • Atsuo Kuroiwa
  • Kenji Ina
  • Kazuhiro Kanayama
  • Takafumi Ando
  • Toshihiro Matsuura
  • Takeo Yamaguchi
  • Kimitomo Morise
  • Mayumi Ieda
  • Hitoshi Iokawa
  • Akira Ishihara
  • Susumu Sarai
Intestinal Disorders, Inflammatory Bowel Disease, Immunology, And Microbiology

DOI: 10.1007/BF02064182

Cite this article as:
Kusugami, K., Fukatsu, A., Tanimoto, M. et al. Digest Dis Sci (1995) 40: 949. doi:10.1007/BF02064182

Abstract

Local interleukin-6 (IL-6) activity was studied using colonic mucosal tissues in inflammatory bowel disease (IBD) and inflammatory control patients. Active IBD specimens exhibited significantly higher IL-6 activity than control specimens in both cultures of isolated lamina propria mononuclear cells (LPMC) and mucosal tissues with an increased number of IL-6-producing cells. However, the activity in inactive IBD or inflammatory controls did not differ from controls. Northern blot analysis demonstrated IL-6 messenger RNA in LPMC and colonic epithelial cells isolated from active IBD specimens but not in control cells. Furthermore, immunofluorescent microscopic study of active IBD specimens showed more conspicuous staining of IL-6 in infiltrating LPMC (mostly CD68+ cells) and colonic epithelial cells. These results suggest that elevation of local IL-6 activity may be a characteristic feature of active IBD and both macrophages and colonic epithelial cells are the major cell types responsible for this phenomenon.

Key words

interleukin-6 inflammatory bowel disease macrophages colonic epithelial cells 

Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Kazuo Kusugami
    • 3
    • 1
    • 2
  • Atsushi Fukatsu
    • 3
    • 1
    • 2
  • Mitsune Tanimoto
    • 3
    • 1
    • 2
  • Masataka Shinoda
    • 3
    • 1
    • 2
  • Jun-Ichi Haruta
    • 3
    • 1
    • 2
  • Atsuo Kuroiwa
    • 3
    • 1
    • 2
  • Kenji Ina
    • 3
    • 1
    • 2
  • Kazuhiro Kanayama
    • 3
    • 1
    • 2
  • Takafumi Ando
    • 3
    • 1
    • 2
  • Toshihiro Matsuura
    • 3
    • 1
    • 2
  • Takeo Yamaguchi
    • 3
    • 1
    • 2
  • Kimitomo Morise
    • 3
    • 1
    • 2
  • Mayumi Ieda
    • 3
    • 1
    • 2
  • Hitoshi Iokawa
    • 3
    • 1
    • 2
  • Akira Ishihara
    • 3
    • 1
    • 2
  • Susumu Sarai
    • 3
    • 1
    • 2
  1. 1.First Department of Internal MedicineAichi Medical UniversityJapan
  2. 2.Daido HospitalNagoyaJapan
  3. 3.First Department of Internal MedicineNagoya University School of MedicineNagoyaJapan

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