Journal of Chemical Ecology

, Volume 20, Issue 3, pp 753–769

Tasting green leaf volatiles by larvae and adults of Colorado potato beetle,Leptinotarsa decemlineata

  • B. K. Mitchell
  • B. G. McCashin
Article
  • 42 Downloads

Abstract

Larvae and adults of the Colorado potato beetle,Leptinotarsa decemlineata (Say), are shown to have galeal gustatory cells that are highly sensitive to distillate of potato leaf extracts, (E)-2-hexen-1-ol, (E)-2-hexenal, and other saturated and unsaturated six-carbon alcohols. In larvae and adults, the sensory response patterns elicited by leaf homogenate, leaf distillate and a mixture of these two extracts differ in subtle ways. Beetle larvae feed most readily on Millipore disks treated with leaf homogenate and the mixture, but they did not feed on disks treated with leaf distillate. The differences in behavioral response and sensory input are used to derive a potential gustatory code that may stimulate different levels of feeding. This code may be disrupted by compounds present in nonhost leaves, thus leading to reduced feeding. Possible interactions of sapid leaf volatiles, amino acids, sugars, and potentially deterrent plant compounds are discussed.

Key Words

Contact chemoreception electrophysiology taste leaf alcohols galeal sensilla feeding behavior host selection gustatory coding Colorado potato beetle Leptinotarsa decemlineata Coleoptera Chrysomelidae 

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References

  1. Bruinsma, J. 1963. The quantitative analysis of chlorophyllsa andb in plant extracts.Photochem. Photobiol. 2:241–249.Google Scholar
  2. Dethier, V.G., andGoldrich Rachman, N. 1976. Anesthetic stimulation of insect water receptors.Proc. Natl. Acad. Sci. U.S.A. 73:3315–3319.PubMedGoogle Scholar
  3. Dickens, J.C. 1984. Olfaction in the boll weevil,Anthonomous grandis BOH. (Coleoptera: Curculionidae): electroantennogram studies.J. Chem. Ecol. 10:1759–1785.Google Scholar
  4. Dickens, J.C. 1989. Green leaf volatiles enhance aggregation pheromone of boll weevil,Anthonomus grandis.Entomol. Exp. Appl. 52:191–203.Google Scholar
  5. Frazier, J.L., andHanson, F.E. 1986. Electrophysiological recording and analysis of insect chemosensory responses, pp. 285–330,in J.R. Miller and T. Miller (eds.). Insect-Plant Interactions. Springer Verlag, New York.Google Scholar
  6. Haley Sperling, J.L., andMitchell, B.K. 1991. A comparative study of host recognition and the sense of taste inLeptinotarsa.J. Exp. Biol. 157:439–459.Google Scholar
  7. Hodgson, E.S., Lettvin, J.Y., andRoeder, K.D. 1955. Physiology of a primary chemoreceptor unit.Science 122:417–418.PubMedGoogle Scholar
  8. Honda, H., Maruyama, Y., andMatsumoto, Y. 1986. Comparisons in EAG response ton-alkyl compounds between the fruit- and Pinaceae-feeding type of yellow peach moth,Conogethes punctiferalis (Guenèe) (Lepidoptera: Pyralidae).Appl. Entomol. Zool. 21:126–133.Google Scholar
  9. Hsaio, T.H., andFraenkel, G. 1968. The influence of nutrient chemicals on the feeding behavior of the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).Ann. Entomol. Soc. Am. 61:44–54.Google Scholar
  10. Light, D.M., andJang, E.B. 1987. Electroantennogram responses of the Oriental fruit fly,Dacus dorsalis, to a spectrum of alcohol and aldehyde plant volatiles.Entomol. Exp. Appl. 45:55–64.Google Scholar
  11. Ma, W.C., andVisser, J.H. 1978. Single unit analysis of odour quality coding by the olfactory antennal receptor system of the Colorado potato beetle.Entomol. Exp. Appl. 24:320–333.Google Scholar
  12. Mitchell, B.K. 1974. Behavioural and electrophysiological investigations on the responses of larvae of the Colorado potato beetle (Leptinotarsa decemlineata) to amino acids.Entomol. Exp. Appl. 17:255–264.Google Scholar
  13. Mitchell, B.K. 1987. Interactions of alkaloids with galeal chemosensory cells of Colorado potato beetle.J. Chem. Ecol. 13:2009–2022.Google Scholar
  14. Mitchell, B.K. 1988. Adult leaf beetles as models for exploring the chemical basis of host plant recognition.J. Insect Physiol. 34:213–225.Google Scholar
  15. Mitchell, B.K. 1993. The chemosensory basis of host-plant recognition in Chrysomelidae, pp. 141–151,in P. Jolivet (ed.). Biology of Chrysomelidae II. W. Junk, Amsterdam.Google Scholar
  16. Mitchell, B.K., andHarrison, G.D. 1984. Characterization of the galeal chemosensilla in the adult Colorado beetle,Leptinotarsa decemlineata.Physiol. Entomol. 9:49–56.Google Scholar
  17. Mitchell, B.K., Rolseth, B.M., andMcCashin, B.G. 1990. Differential responses of galeal sensilla of the adult Colorado potato beetle,Leptinotarsa decemlineata (Say), to leaf saps from host and non-host plants.Physiol. Entomol. 15:61–72.Google Scholar
  18. Mitchell, B.K., andSchoonhoven, L.M. 1974. Taste receptors in Colorado beetle larvae.J. Insect. Physiol. 20:1787–1793.PubMedGoogle Scholar
  19. Sen, A., andMitchell, B.K. 1987. Ultrastructure of the galeal sensory complex in adults of the Colorado potato beetle,Leptinotarsa decemlineata.Physiol. Entomol. 12:81–90.Google Scholar
  20. Smith, J.J.B., Mitchell, B.K., Rolseth, B.M., Whitehead, A.T., andAlbert, P.J. 1990. SAPID Tools: Microcomputer programs for analysis of multi-unit nerve recordings.Chem. Senses 15:253–270.Google Scholar
  21. Staedler, E., andHanson, F.E. 1975. Olfactory capabilities of the “gustatory” chemoreceptors of the tobacco hornworm larvae.J. Comp. Physiol. 104:97–102.Google Scholar
  22. Visser, J.H. 1979. Electroantennogram responses of the Colorado potato beetle,Leptinotarsa decemlineata, to plant volatiles.Entomol. Exp. Appl. 25:86–97.Google Scholar
  23. Visser, J.H. 1986. Host odor perception in phytophagous insects.Annu. Rev. Entomol. 31:121–144.Google Scholar
  24. Visser, J.H., andAve, D.A. 1978. General green leaf volatiles in the olfactory orientation of the Colorado potato beetle,Leptinotarsa decemlineata.Entomol. Exp. Appl. 24:738–749.Google Scholar
  25. Visser, J.H., van Straten, S., andMaarse, H. 1979. Isolation and identification of volatiles in the foliage of potato,Solanum tuberosum, a host plant of the Colorado potato beetle,Leptinotarsa decemlineata.J. Chem. Ecol. 5:11–23.Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • B. K. Mitchell
    • 1
  • B. G. McCashin
    • 1
  1. 1.Department of EntomologyUniversity of AlbertaEdmontonCanada

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