Journal of Chemical Ecology

, Volume 20, Issue 4, pp 929–942

Influence of cnicin, a sesquiterpene lactone ofCentaurea maculosa (Asteraceae), on specialist and generalist insect herbivores

  • I. Landau
  • H. Müller-Schärer
  • P. I. Ward


The sesquiterpene lactone cnicin was extracted fromCentaurea maculosa andCentaurea vallesiaca. We examined its effects on the ovipositional response and larval development of generalist and specialist insect herbivores associated withC. maculosa. For the oviposition trials, three plant species (C. maculosa, Achillea millefolium, andCichorium intybus), half of which were sprayed with 3% of cnicin, were exposed to the specialist mothsStenodes straminea, Agapeta zoegana, andPterolonche inspersa in field cages. All three species significantly preferredC. maculosa to other plants andP. inspersa significantly preferred cnicin-sprayed plants to untreated plants for oviposition. Tested over all species, cnicin significantly increased the number of eggs laid on a given plant. A larval diet test examined the toxicity of cnicin for larvae of the generalist noctuid mothSpodoptera littoralis. Cnicin concentrations of 3% and 6% were lethal and 1% and 0.5% seriously inhibited growth and development. The larvae of theC. maculosa specialistStenodes straminea survived at 6% cnicin, but none of the pupae hatched.Agapeta zoegana was able to survive at 1% and 3% cnicin. Both specialists had difficulties with the artificial diet, but weight increase and survival was not further reduced when cnicin was present compared with on the control diet. In conclusion, cnicin influenced host recognition by the specialist species, and larvae of the generalist did not survive on natural levels of cnicin. Growth and survival of the specialist were not influenced by cnicin but were considerably hampered on artificial diet.

Key Words

Centaurea maculosa sesquiterpene lactone cnicin host-plant selection oviposition behavior antifeedant attractant Spodoptera littoralis Agapeta zoegana Stenodes straminea Pterolonche inspersa Lepidoptera Noctuidae Cochylidae Pterolonchidae 


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  1. Bloszyk, E. andDrozdz, B. 1978. Part XXII. Sesquiterpene lactones in species of the genusChrysanthemum.Acta Soc. Bot. Polon. 47 (1–2):3–12.Google Scholar
  2. Burnett, W.C., Jones, S.B., Mabry, T.J., andPadolina, W.G. 1974. Sesquiterpene lactones—insect feeding deterrents inVernonia.Biochem. Syst. Ecol. 2:25–29.Google Scholar
  3. Burnett, W.C., Jones, S.B., andMabry, T.J. 1987. The role of sesqiterpene lactones in plant-animal coevolution.Annu. Proc. Phytochem. Soc. Eur. 15:233–257.Google Scholar
  4. Dunn, P.H., Rosenthall, S.S., Campobasso, G., andTait, S.M. 1989. Host specificity ofPterolonche inspersa (Lep.: Pterolonchidae) and its potential as a biological control agent forCentaurea diffusa, diffuse knapweed, andC. maculosa, spotted knapweed.Entomophaga 34(4):435–446.Google Scholar
  5. Feeny, P. 1976. Plant apparency and chemical defense. Biochemical interactions between plants and insects.Recent Adv. Phytochem. 10:1–40.Google Scholar
  6. Fischer, N.H., Olivier, E.J., andFischer, H.D. 1979. The biogenesis and chemistry of sesquiterpene lactones.Prog. Chem. Org. Nat. Prod. 38:47–390.Google Scholar
  7. Geppert, B., Drozdz, B., Kielczewski, M. andHolub, M. 1983. Sesquiterpene lactones. XXIII. Isolation of sesquiterpene lactones fromCentaurea L. species.Acta Soc. Bot. Polon. 52(1):23–34.Google Scholar
  8. Harmatha, J. andNawrot, J. 1984. Comparison of the feeding deterrent activity of some sesquiterpene lactones and a lignan lactone towards selected insect storage pests.Biochem. Syst. Ecol. 12(1):95–98.Google Scholar
  9. Huneck, S., Jakupovic, J., andSchuster, A. 1986. Weitere Inhaltsstoffe ausCentaurea stoebe.Planta Med. 5:398–399.Google Scholar
  10. Jones, S.B., Burnett, W.C., Coile, N.C., Mabry, T.J., andBetouski, M.F. 1979. Sesqiterpene lactones ofVernonia—influence of glaucolide-A on the growth rate and survival of lepidopterous larvae.Oecologia 39:71–77.Google Scholar
  11. Kelsey, R.G. andLocken, L.J. 1987. Phytotoxic properties of cnicin, a sesquiterpene lactone fromCentaurea maculosa (spotted knapweed).J. Chem. Ecol. 13(1):19–33.Google Scholar
  12. Kery, A., Turiak, G.V., Zambo, I., andTetenyi, P. 1987. Comparison of different separation techniques on the biologically active sesquiterpene lactones.Acta Pharm. Hung. 57:228–238.PubMedGoogle Scholar
  13. Landau, I. 1993. Cnicin, ein Sesquiterpenlacton der gefleckten FlockenblumeCentaurea maculosa: Einfluss auf Spezialisten- und Generalisten-Herbivoren. Diploma thesis. University of Zürich.Google Scholar
  14. Locken, L.J. andKelsey, R.G. 1987. Cnicin concentrations inCentaurea maculosa, spotted knapweed.Biochem. Syst. Ecol. 15(3):313–320.Google Scholar
  15. Mabry, T.J. andGill, J.E. 1979. Sesquiterpene lactones and other terpeniods, pp. 502–537,in G.A. Rosenthal and D.H. Janzen (eds.). Herbivores: Their Interaction with Secondary Plant Metabolites. Academic Press, New York.Google Scholar
  16. Mabry, T.J., Gill, J.E., Burnett, W.C., andJones, S.B. 1977. Antifeedant sesquiterpene lactones in the Compositae, pp. 179–184,in P.A. Hedin (ed.). Host Plants Resistance to Pests. American Chemical Society, Washington.Google Scholar
  17. Muir, A.D. andMajak, W. 1983. Allelopathic potential of diffuse knapweed (Centaurea diffusa) extracts.Can. J. Plant Sci. 63:989–996.Google Scholar
  18. Muir, A.D., Majak, W., Balza, F., andTowers, G.H.N. 1985. A search for the allelopathic agents in diffuse knapweed. A.C.S. Symposium Series 330. Allochemicals: Role in Agriculture and Forestry. American Chemical Society, Washington, D.C.Google Scholar
  19. Müller, H. 1983. Untersuchungen zur Eignung vonStenodes straminea Haw. (Lep: Cochylidae) für die biologische Bekämpfung vonCentaurea maculosa Lam. (Gefleckte Flockenblume) (Compositae) in Kanada.Bull. Soc. Entomol. Suisse 56:329–342.Google Scholar
  20. Müller, H. 1989. Structural analysis of the phytophagous insect guilds associated with the roots ofCentaurea maculosa Lam.,C. diffusa Lam., andC. vallesiaca Jordan in Europe.Oecologia 78:41–52.Google Scholar
  21. Müller, H. andSteinger, T. 1990. Separate and joint effects of root herbivores, plant competition and nitrogen shortage on resource allocation and components of reproduction inCentaurea maculosa (Compositae).Symp. Biol. Hung. 39:215–224.Google Scholar
  22. Müller, H., Schroeder, D., andGassmann, A. 1988.Agapeta zoegana (L.) (Lep: Cochylidae), a suitable prospect for biological control of spotted and diffuse knapweed,Centaurea maculosa Monnet de la Marck andCentaurea diffusa Monnet de la Marck (Compositae) in North America.Can. Entomol. 120:109–124.Google Scholar
  23. Müller-Schärer, H. andSchroeder, D. 1993. The biological control ofCentaurea spp. in North America: Do insects solve the problem?Pestic. Sci. 37:343–353.Google Scholar
  24. Navon, A. 1985.Spodoptera littoralis P. Sigh and R.F. Moore (eds.). Handbook of Insect Rearing, Vol. II. Elsevier, Amsterdam.Google Scholar
  25. Nawrot, J., Smitalova, Z., andHolub, M. 1983. Deterrent activity of sesquiterpene lactones from the Umbelliferae against storage pests.Biochem. Syst. Ecol. 11(3):243–245.Google Scholar
  26. Nowak, G., Drozdz, B., andGeorgiadis, T. 1984. Sesquiterpene lactones. XXIX. Cnicin in species of the subgenusAcrolophus (Cass.)Dobrocz. Acta Soc. Bot. Polon. 53(2):199–205.Google Scholar
  27. Picman, A.K. andPicman, J. 1984. Effect of selected pseudoguaianolides on survival of the flour beetle,Tribolium confusum.Biochem. Syst. Ecol. 12(1):89–93.Google Scholar
  28. Picman, A.K., Elliott, R.H., andTowers, G.H.N. 1978. Insect feeding deterrent property of alantolactone.Biochem. Syst. Ecol. 6:333–335.Google Scholar
  29. Rodriguez, E., Towers, G.H.N., andMitchell, J.C. 1976. Biological activities of sesquiterpene lactones.Phytochemistry 15:1573–1580.Google Scholar
  30. Schroeder, D. 1985. The search for effective biological control agents in Europe. 1. Diffuse and spotted knapweed, pp. 103–119,in E.S. Delfoss (ed.). Proc. 6th Int. Symp. Biol. Contr. Weeds, Vancouver, B.C., Canada, 1984. Agriculture Canada.Google Scholar
  31. Seaman, F.C. 1982. Sesquiterpene lactones as taxonomic characters in the Asteraceae.Bot. Rev. 48(2):121–595.Google Scholar
  32. Sokal, R.R. andRohlf, F.J. 1981. The Principles and Practice of Statistics in Biological Research, 2nd ed. W.H. Freeman, New York.Google Scholar

Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • I. Landau
    • 1
    • 2
  • H. Müller-Schärer
    • 1
  • P. I. Ward
    • 2
  1. 1.Swiss Federal Research StationWädenswilSwitzerland
  2. 2.Zoological Museum of the University of ZürichZürichSwitzerland

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