Advertisement

Mycopathologia

, Volume 28, Issue 3, pp 197–205 | Cite as

Some physiological studies in two species of phytophthora

  • Vasant Gurunath Rao
  • M. K. Desai
  • N. B. Kulkarni
Article

Summary

The paper deals with some physiological studies viz., (1) Production of enzymes (2) Effect of chemicals and sugar solutions and (3) Utilization of amino acids, by two species ofPhytophthora i.e.,Phytophthora palmivoraButler andP. parasiticaDast. var.macrosporaAshby., isolated from rotten fruits ofAchras sapota andAnona squamosa respectively.

In the study of production of extra-cellular enzymes, it was found that both the isolates ofPhytophthora produced enzymes like inulase, amidase, emulsin and diastase in small quantity.

Among the various chemicals and salt solutions used to study their effects in the formation of sporangia; it was observed that these were remarkably produced in abundance by both the species ofPhytophthora specially in the chemical solutions like sodium chloride, potassium nitrate, sodium thiosulphate and potassium permanganate. On the other hand, chemical solutions like strontium sulphate, potassium chromate, ammonium oxalate, zinc sulphate, copper sulphate, potassium sulphate, strontium nitrate and ferrous sulphate completely ceased the sporangial development. Among the various sugar solutions tried, dextrose, galactose, glucose, laevulose and fructose accelerated sporangial formation.

An attempt was also made to study the effect of various amino acids singly or in combination on growth and sporulation. Both the isolates under study, utilized L-Arganine monohydrochloride and DL-Aspartic acid, hence these were growth promoters. It is interesting to note that the growth of the isolates was good, only when DL-Norleucine and DL-Methionine (both growth inhibitors) were provided in combination.

Keywords

Phytophthora Sugar Solution Thiosulphate Potassium Permanganate Ferrous Sulphate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Crabill, C. H. &Reed, H. S. (1915). Convenient methods of demonstrating the biochemical activity of the micro-organisms with special reference to the production of enzymes. Biochem. Bull.4 30–44.Google Scholar
  2. Gooding, G. V. &Lucas, G. B. (1959). Factors influencing sporangial formation and zoopores activity inPhytophthora parasitica. Phytopathology49 277–281.Google Scholar
  3. Leonian, L. H. &Lilly, V. G. (1940). Studies on the nutrition of fungi IV. Factors influencing the growth of some thiamin-requiring fungi. Amer. J. Bot.27 18–26.CrossRefGoogle Scholar
  4. Lewis, R. W. (1952). The Vitamin nutrition ofAlternaria solani. Phytopathology42 657–659.Google Scholar
  5. Lewis, R. W. (1957). Amino acid nutrition ofAlternaria solani. Phytopathology47 121–124.Google Scholar
  6. Lilly, V. G. &Barnett, H. L. (1951). Physiology of the Fungi. McGraw Hill Book Co. Inc. New York & London.Google Scholar
  7. Lindeberg, G. (1944). Ueber die Physiologie Ligninabbauender Bodenhymenomyzeten. Symb. Bot. Upsalienses8 2: 1–183.Google Scholar
  8. McKay, R. (1937). “Potassium permanganate” as an aid to the production of sexual fruitifications byPhytophthora erythroseptica Pethybr. Abs. in Rev. appl. Mycol.16 701.Google Scholar
  9. Rao, V. G. (1960). Fruit rots of Chiku (Achras sapota L.) and Sitaphal (Anona squamosa L.) two hitherto unrecorded diseases in Bombay State; caused byPhytophthora palmivora Butler andPhytophthora parasitica Dastur var.macrospora Ashby respectively. M. Sc. (Agri.) Thesis, pp. 96, Plates 11, University of Poona, (unpublished).Google Scholar
  10. Rao, V. G., Desai, M. K. &Kulkarni, N. B. (1962a). A newPhytophthora fruit rot ofAchras sapota from India. Plant. Dis. Reptr.46 5: 381–382.Google Scholar
  11. Rao, V. G., Desai, M. K. &Kulkarni, N. B. (1962b). A newPhytophthora fruit rot ofAnona squamosa from India. Ibid.46 12: 874–876.Google Scholar
  12. Thom, C. &Raper, K. B. (1945). Manual of Aspergilli. Williams and Willkins Co., Baltimore.Google Scholar

Copyright information

© Dr. W. Junk N.V. 1966

Authors and Affiliations

  • Vasant Gurunath Rao
    • 1
  • M. K. Desai
    • 1
  • N. B. Kulkarni
    • 1
  1. 1.Plant Pathology LaboratoriesCollege of AgriculturePoona-5India

Personalised recommendations