Hyperfine Interactions

, Volume 40, Issue 1–4, pp 147–157

Protein structural dynamics as determined by Mössbauer spectroscopy

  • Fritz Parak
  • Joachim Heidemeier
  • Gerd U. Nienhaus
Invited Papers, Concluding Remarks

Abstract

Mössbauer spectroscopy on57Fe allows the study of dynamics with a characteristic time faster 100 ns. For myoglobin a detailed physical picture of protein dynamics has been obtained. A myoglobin molecule has no well defined energy minimum. X-ray structure analysis yields only an average conformation. At low temperatures the molecules are trapped in slightly different structures called conformational substates. At higher temperatures a Brownian type of oscillation of molecular segments in restricted space occurs. RSMR technique allows an estimation of the characteristic size of these segments which are in myoglobin well below 30 A and larger than 6 A. A determination of the quasielastic absorption with high accuracy yields the energy distribution of the conformational substates. As further examples bacteriorhodopsin and a model compound for membranes are discussed.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. /1/.
    Gonser U. and Grant R.W. (1965), Biophys. J.5, 823–844Google Scholar
  2. /2/.
    Parak F. and Formanek H. (1971), Acta Cryst.,27A, 573–578Google Scholar
  3. /3/.
    Mössbauer R.L. (1985) in: Applications of the Mössbauer Effect, ed. Yu.M. Kagan and I.S. Lyubutin, Vol. 1, Gordon and BreachGoogle Scholar
  4. /4/.
    Mössbauer R.L. (1987), Hyperfine Interactions33, 199–222Google Scholar
  5. /5/.
    Dwivedi A., Pederson T. and Debrunner P.G. (1979), J. de PhysiqueC2, 531–533Google Scholar
  6. /6/.
    Parak F. (1980), J. de Physique41, Coll. Cl, 71–78Google Scholar
  7. /7/.
    Krupyanskii Yu.F., Parak F., Gaubmann E.E., Wagner F.M., Goldanskii V.I., Mössbauer R.L.; Suzdalev I.P., Litterst F.J. and Vogel H. (1980), J. de Physique41, Coll. Cl, 489–490Google Scholar
  8. /8/.
    Bauminger E.R., Cohen S.G., Novik I., Ofer S and Yariv J. (1982), in: Int. Conf. on the Appl. of the Mössbauer Effect, ed. V.G. Bhide, Indian National Science Academy, New Delhi, pages 640–644Google Scholar
  9. /9/.
    Goldanskii V.I. (1985) in: Applications of the Mössbauer Effect, ed. Yu.M. Kagan and I.S. Lyubutin, Vol. 1, Gordon and BreachGoogle Scholar
  10. /10/.
    Parak F. (1985) in: Applications of the Mössbauer Effect, ed. Yu.M. Kagan and I.S. Lyubutin, Vol. 1, Gordon and BreachGoogle Scholar
  11. /11/.
    Albanese G., Deriu A., Cavatorta F., Krupyanskii Yu.F., Suzdalev I.P. and Goldanskii V.I. (1986), Hyperfine Interactions29, 1407–1409Google Scholar
  12. /12/.
    Nienhaus G.U. and Parak F. (1986), Hyperfine Interactions29, 1451–1454Google Scholar
  13. /13/.
    Bauminger E.R., May L., Nowik I. and Heitner-Wirguin C. (1986), Hyperfine Interactions29, 1335–1338Google Scholar
  14. /14/.
    Parak F., Knapp E.W. and Kucheida D. (1982), J. Mol. Biol.161, 177–194Google Scholar
  15. /15/.
    Bauminger E.R., Cohen S.G., Nowik, I., Ofer S. and Yariv J. (1983), Proc. Natl. Acad. Sci. USA80, 736–740Google Scholar
  16. /16/.
    Knapp E.W., Fischer S.F. and Parak F. (1983), J. Chem. Phys.78, 4071–4711Google Scholar
  17. /17/.
    Nowik I., Cohen S.G., Bauminger E.R. and Ofer S. (1983), Phys. Rev. Lett.50, 1528–1531Google Scholar
  18. /18/.
    Nadler W. and Schulten K. (1983), Phys. Rev. Lett.51, 1712–1715Google Scholar
  19. /19/.
    Nadler W. and Schulten K. (1984), Proc. Natl. Acad. Sci. USA81, 5719–5723Google Scholar
  20. /20/.
    Parak F., Hartmann H., Aumann K.D., Reuscher H., Rennekamp G., Bartunik H. and Steigemann W. (1987), Eur. Biophys. J., in pressGoogle Scholar
  21. /21/.
    Knapp E.W., Fischer S.F. and Parak F. (1982), J. Phys. Chem.86, 5042–5047Google Scholar
  22. /22/.
    Parak F., Hartmann H., Nienhaus G.U. and Heidemeier J. (1987), in: Structure, Dynamics and Function of Biomolecules, ed.: A. Ehrenberg, R. Rigler, A. Gräslund, and L. Nilsson, Springer Verlag, pages 30–33Google Scholar
  23. /23/.
    Austin R. H., Beeson K.W., Eisenstein L., Frauenfelder H. and Gunsalus I.C. (1975), Biochem.14, 5355–5373Google Scholar
  24. /24/.
    Frauenfelder H., Petsko G.A. and Tsernoglou D. (1979), Nature (London)280, 558–563Google Scholar
  25. /25/.
    Hartmann H., Parak F., Steigemann W., Petsko G.A., Ponzi D.R. & Frauenfelder H. (1982), Proc. Natl. Acad. Sci., USA79, 4967–4971Google Scholar
  26. /26/.
    Ansari A., Berendzen J., Bowne S.F., Frauenfelder H., Iben I.E.T., Sauke T.B., Shyamsunder E., Young R.D. (1985), Proc. Natl. Acad. Sci. USA82, 5000–5004Google Scholar
  27. /27/.
    Parak F. and Knapp E.W. (1984), Proc. Natl. Acad. Sci. USA81, 7088–7092Google Scholar
  28. /28/.
    Parak F., Fischer M., Graffweg E. and Formanek H. (1987), in: Structure and Dynamics of Nucleic Acids, Proteins and Membranes, ed.: E. Clementi and S. Chin, Plenum Publish. Comp. New York, 139–148Google Scholar
  29. /29/.
    Krupjanskii Yu., Parak F., Goldanskii V.I., Mössbauer R.L., Gaubmann E.E., Engelmann H. and Suzdalev I.P. (1982), Z. Naturforschg.37 c, 57–62Google Scholar
  30. /30/.
    Parak F., Frolov E.N., Kononenko A.A., Mössbauer R.L., Goldanskii V.I. and Rubin A.B. (1980), FEBS lett.117, 368–372Google Scholar
  31. /31/.
    Heidemeier J., Fischer M., Parak F., Engelhard M., Hess B., Formanek H., in preparationGoogle Scholar
  32. /32/.
    Reinisch L., Heidemeier J. and Parak F. (1985), Eur. Biophys. J.12, 167–172Google Scholar

Copyright information

© J.C. Baltzer AG, Scientific Publishing Company 1988

Authors and Affiliations

  • Fritz Parak
    • 1
  • Joachim Heidemeier
    • 1
  • Gerd U. Nienhaus
    • 1
  1. 1.Institut für Physikalische Chemie der UniversitätMünsterFed. Rep. of Germany

Personalised recommendations