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Antonie van Leeuwenhoek

, Volume 30, Issue 1, pp 225–238 | Cite as

Sulphur metabolism in Thiorhodaceae I. Quantitative measurements on growing cells ofChromatium okenii

  • H. G. Trüper
  • H. G. Schlegel
Article

Abstract

Growth experiments and short term experiments in a stirred cuvette showed thatChromatium okenii strain Ostrau is not able to oxidize any reduced sulphur compounds except sulphide and elementary sulphur; thiosulphate, sulphite, and thioglycolate can not be utilized as reducing agents for photosynthesis. The cells are not able to use H2; hydrogenase could not be demonstrated. In the dark, sulphide is formed from intracellular sulphur and the carbon content of the cells decreases. Growth and turnover of sulphur compounds was followed in the light in the presence and absence of acetate as a second carbon source. Sulphide oxidation depends on the presence of CO2 and on light intensity, i.e. sulphur metabolism is governed by the photosynthetic activity of the cells.

Keywords

Sulphur Acetate Sulphide Carbon Source Light Intensity 
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Copyright information

© Swets & Zeitlinger 1964

Authors and Affiliations

  • H. G. Trüper
    • 1
  • H. G. Schlegel
    • 1
  1. 1.Institute of MicrobiologyThe UniversityGöttingenGermany

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