Antonie van Leeuwenhoek

, Volume 31, Issue 1, pp 301–313 | Cite as

Tolerance ofChlorella vulgaris for metallic and non-metallic ions

  • L. E. den Doore de Jong
  • W. B. Roman


The well-known, extreme sensitivity of algae towards Cu++ ions prompted a systematic investigation of the tolerance ofChlorella vulgaris for both metallic (49) and non-metallic (7) ions. With thirty metals forming weak bases, pH effects were to some extent super-imposed on the toxic effects of the metal ions themselves. With the elements U, Zr, V and Sb, oxy-compounds had to be used. The elements Mo, W and Bi were tested as components of anions.

From the metals that form strong bases, the salts of Na, K, Rb, Ca, Sr and Mg were tolerated in high concentrations; the maximum values of these ranged from 0.11 – 0.98 g at/liter. Notwithstanding some unavoidable simplifications of the experimental technique, it could be concluded from the results that in four intermetal groups, arranged according to I.U.P.A.C., toxicity has a definite tendency to increase with increasing atomic number. This held for the series: Na, K, Rb, Cs; Mg, Ca, Sr, Ba; Zn, Cd, Hg; Al, In, Tl. In like manner, the rare earths were found to be more toxic than the alkaline earth metals.

Co, Ni and Cu completely inhibit growth at very low concentrations ranging from 4.2×10−6−2×10−5 g at/liter; in view of the relatively low atomic numbers of these metals, the toxicity must be regarded as specific (algotoxicity).

Among the non-metals, Sb and As proved highly toxic. Fluoride ions were considerably more toxic than chloride and bromide ions.


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  1. Bartsch, A. F. 1954. Practical methods for control of algae and water weeds. Public Health Rept. (U.S.)69: 749–757.Google Scholar
  2. Cooper, L. H. N. 1948. Some chemical considerations on the distribution of iron in sea water. J. Marine Biol. Assoc. U.K.27: 314–321.CrossRefGoogle Scholar
  3. Emerson, R. andLewis, C. M. 1939. Factors influencing the efficiency of photosynthesis. Am. J. Botany26: 802–822.Google Scholar
  4. Greenfield, S. S. 1942. Inhibitory effects of inorganic compounds on photosynthesis inChlorella. Am. J. Botany29: 121–131.Google Scholar
  5. Ichioka, P. S. andArnon, D. I. 1955. Molybdenum in relation to nitrogen metabolism. II. Assimilation of ammonia and urea without molybdenum byScenedesmus obliquus. Physiol. Plantarum8: 552–560.Google Scholar
  6. Katoh, S. 1960. A new copper protein fromChlorella ellipsoidea. Nature186: 533–534.PubMedGoogle Scholar
  7. Kellner, K. 1955. Die Adaptation vonAnkistrodesmus braunii an Rubidium und Kupfer. Biol. Zentr.74: 662–691.Google Scholar
  8. Lewin, R. A. 1962. Physiology and biochemistry of Algae. Academic Press, New York.Google Scholar
  9. Löhnis, M. P. 1946. Plantenvoeding, Noorduijn, Gorinchem.Google Scholar
  10. Stotz, E., Harrer, C. J. andKing, C. G. 1937. A study of “ascorbic acid oxidase” in relation to copper. J. Biol. Chem.119: 511–522.Google Scholar
  11. Walker, J. B. 1953. Inorganic micronutrient requirements ofChlorella. I. Requirements for calcium (or strontium), copper and molybdenum. Arch. Biochem. Biophys.46: 1–11.CrossRefPubMedGoogle Scholar
  12. Walker, J. B. 1954. Inorganic micronutrient requirements ofChlorella. II. Quantitative requirements for iron, manganese and zinc. Arch. Biochem. Biophys.53: 1–8.CrossRefGoogle Scholar
  13. Young, R. S. 1935. Certain rarer elements in soils and fertilizers and their role in plant growth. Cornell Univ. Agr. Expt. Sta. Mem. No. 174.Google Scholar

Copyright information

© Swets & Zeitlinger, Publishers 1965

Authors and Affiliations

  • L. E. den Doore de Jong
    • 1
  • W. B. Roman
  1. 1.Central Bacteriological Laboratory of the Municipal HospitalsRotterdamThe Netherlands

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