Advertisement

Acta diabetologia latina

, Volume 18, Issue 4, pp 329–334 | Cite as

Differentiation of A-, B- and D-cells of guinea-pig pancreas using semi-thin epoxy-resin sections

  • James McGadey
  • Owen Reid
Original Contributions

Summary

Four-mm2 specimens of guinea-pig pancreas were removed and fixed in buffered 5% glutaraldehyde, dehydrated through alcohols, treated with propylene oxide and impregnated with epoxyresin and finally polymerised at 70 °C. One-μ sections were cut on an ultramicrotome, stretched and adhered to glass slides. They were then deresinized in saturated sodium ethoxide, hydrated and washed thoroughly in running water. Sections were then stained by hematoxylin and eosin, Masson, Mallory, Epple, McGadey and the hematoxylin/acid fuchsin/orange G/soluble blue routines using the modified Weigert hematoxylin for the nuclear staining. They were then dehydrated, cleared and mounted in D.P.X. The stainings of the islet cells were compared and only by the latter routine did the D-cells stain adequately.

Key-words

A-cells B-cells D-cells Differentiation Epoxy-resin Histology Pancreatic islets Semithin Sodium ethoxide Viscosity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1).
    Aparicio S. R., Marsden P.: A rapid methylene blue-basic fuchsin stain for semi-thin sections of peripheral nerve and other tissues - J. Microsc. (Oxford)89, 139, 1969.Google Scholar
  2. 2).
    Aparicio S. R., Marsden P.: Application of standard micro-anatomical staining methods to epoxy resin-embedded sections - J. clin. Path.22, 589, 1969.PubMedGoogle Scholar
  3. 3).
    Bailey G. W. (Ed.): Rapid infiltration of biological tissues utilising n-hexenyl succinic anhydride (HXSA) vinyl cyclohexene dioxide (VCD) an ultra-low viscosity embedding medium - 34th Ann. Proc. Electr.-Microsc. Soc. Amer., Claitors Publishing Division, Baton Rouge-Los Angeles, 1976; p. 346.Google Scholar
  4. 4).
    Beccari N.: Elementi di tecnica microscopica - Soc. Ed. Libraria, Milano 1952; p. 243.Google Scholar
  5. 5).
    Bennett S. H., Wyrick A. D., Lee S. W., McNeil J. H.: Science and art in preparing tissue embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains - Stain Technol.51, 71, 1976.PubMedGoogle Scholar
  6. 6).
    Bensley R. R.: Studies on the pancreas of the guinea pig - Amer. J. Anat.12, 297, 1911.CrossRefGoogle Scholar
  7. 7).
    Bloom W.: A new type of granular cell in the islets of Langerhans of man - Anat. Rec.49, 363, 1931.CrossRefGoogle Scholar
  8. 8).
    Chang S. C.: Hematoxylin and eosin staining of plastic embedded sections - Arch. Path.93, 344, 1972.PubMedGoogle Scholar
  9. 9).
    Epple A.: A staining sequence for A-, B-, and D-cells of the pancreatic islets - Stain Technol.42, 53, 1967.PubMedGoogle Scholar
  10. 10).
    Glauert A. M., Glauert R. H.: Araldite as an embedding medium for electron microscopy - J. biophys. biochem. Cytol.4, 191, 1958.PubMedGoogle Scholar
  11. 11).
    Glauert A. M., Rogers G. E., Glauert R. H.: A new embedding medium for electron microscopy - Nature (Lond.)178, 803, 1956.Google Scholar
  12. 12).
    Gomori G.: Studies of the cells of pancreatic islets - Anat. Rec.74, 439, 1939.CrossRefGoogle Scholar
  13. 13).
    Grimley P. M., Albrecht J. M., Michelitch H. J.: Preparation of large sections for light microscopy as an adjunct to fine-structure studies - Stain Technol.40, 357, 1965.PubMedGoogle Scholar
  14. 14).
    Hoff A. F., Rayburn C.: A modified alcian blue-periodic acid Schiff stain for epoxy-embedded atherosclerotic arteries - Stain Technol.40, 241, 1974.Google Scholar
  15. 15).
    Humber J. D., Parker F., Odland G. F.: A basic fuchsin and alkalinized methylene blue rapid stain for epoxy embedded tissue - Stain Technol.43, 83, 1968.PubMedGoogle Scholar
  16. 16).
    Kito H., Hosoda S.: Triple staining for simultaneous visualization of cell types in islets of Langerhans of pancreas - J. Histochem. Cytochem.25, 1019, 1977.PubMedGoogle Scholar
  17. 17).
    Lane B. P., Europa D. L.: Differential staining of ultra-thin sections on epoxy-embedded tissue for light microscopy - J. Histochem. Cytochem.13, 579, 1965.PubMedGoogle Scholar
  18. 18).
    Lumsden C. E.,Aparicio S. R.: The preparation of ultra-thin sections for light microscopy - J. clin. Path. (ACP) Broadsheet 72, Dec. 1970.Google Scholar
  19. 19).
    Maldonado R., San José H.: A phloxine-azur-hematoxylin sequence for differential staining of cells in pancreatic islets - Stain Technol.42, 215, 1967.Google Scholar
  20. 20).
    Mayor H. D., Hampton J. C., Rosario B.: A simple method for removing the resin from epoxy embedded tissue - J. biophys. biochem. Cytol.9, 909, 1961.PubMedGoogle Scholar
  21. 21).
    McGadey J.: Bulk staining method for alpha and beta cells of the pancreatic islets - J. med. Lab. Technol.27, 310, 1970.PubMedGoogle Scholar
  22. 22).
    McGadey J.: A staining sequence for the differentiation of A-, B-, and D-cells of the islets of guinea-pig pancreas - Acta diabet. lat.16, 243, 1979.Google Scholar
  23. 23).
    Pool C. R.: Hematoxylin and eosin staining of OsO4fixed epoxy-embedded tissue, prestaining oxidation by acidified H2O2 - Stain Technol.44, 75, 1969.PubMedGoogle Scholar
  24. 24).
    Pool C. R.: Prestaining oxidation by acidified H2O2 for revealing Schiff-positive sites in epoxyembedded sections - Stain Technol.48, 123, 0000.Google Scholar
  25. 25).
    Rona G., Morvay I.: Differentiation of cells in the hypophysis and pancreatic islets - Stain Technol.31, 215, 1956.PubMedGoogle Scholar
  26. 26).
    Sato R., Shamoto M.: A simple rapid polychrome stain for epoxy-embedded tissue - Stain Technol.48, 223, 1973.PubMedGoogle Scholar
  27. 27).
    Schweisthal M. R., Frost C. C., Brinn J. E.: Stains for A, B and D-cells in fetal rat islets - Stain Technol.50, 161, 1975.PubMedGoogle Scholar
  28. 28).
    Spurr A. R.: A low viscosity epoxy resin embedding medium for electron microscopy - J. Ultrastruct. Res.26, 31, 1969.CrossRefPubMedGoogle Scholar

Copyright information

© Casa Editrice 1981

Authors and Affiliations

  • James McGadey
    • 1
  • Owen Reid
    • 1
  1. 1.Anatomy DepartmentUniversity of GlasgowGlasgow

Personalised recommendations