Lasers in Medical Science

, Volume 4, Issue 2, pp 115–123

Tumour marking properties of different haematoporphyrins and tetrasulfonated phthalocyanine—A comparison

  • S. Andersson-Engels
  • J. Ankerst
  • J. Johansson
  • K. Svanberg
  • S. Svanberg
Article

Abstract

Several photosensitizers were screened for their tumour-marking ability using laserinduced fluorescence in Wistar/Furth rats bearing subcutaneous adenocarcinomas inoculated in muscle. Of the studied photosensitizers, dihaematoporphyrin ether appeared to exhibit the best tumour-demarcation properties. Polyhaematoporphyrin ester and tetrasulfonated phthalocyanine were almost as good although the fluorescence yield was much lower. Monomeric haematoporphyrin also showed some tumour-marking qualities. By forming fluorescence intensity ratios, information from both the blue and the red spectral regions were used to provide the highest tumour-to-muscle contrast. Two excitation wavelengths were used, of which 337 nm rather than 405 nm excitation light seemed to yield a better tumour demarcation, due to a greater difference in the superimposing autofluorescence between tumour and surrounding tissue. The study included measurements on many inner organs in an attempt to gain a better understanding of the interaction between the drugs and various kinds of tissue.

Key words

Cancer detection Fluorescence spectroscopy Haematoporphyrin derivative Laser excitation Phthalocyanine 

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Copyright information

© Baillière Tindall 1989

Authors and Affiliations

  • S. Andersson-Engels
    • 1
  • J. Ankerst
    • 1
    • 2
  • J. Johansson
    • 1
  • K. Svanberg
    • 1
    • 2
    • 3
  • S. Svanberg
    • 1
  1. 1.Department of PhysicsLund Institute of TechnologyLund
  2. 2.Department of MedicineLund University HospitalLundSweden
  3. 3.Department of OncologyLund University HospitalLundSweden

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