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Lasers in Medical Science

, Volume 1, Issue 1, pp 5–12 | Cite as

Porphyrin-sensitized photodynamic inactivation of cells: A review

  • Johan Moan
Article

Abstract

When mammalian cells are exposed to light in the presence of photosensitizers, such as porphyrins, a number of reactions takes place. The most important photoproduct is singlet oxygen, which reacts with and degrades a variety of biomolecules. This species can travel only about 0.1μm in a cell during its lifetime. Therefore, the structures most exposed to photodamage are those containing high concentrations of sensitizer. For porphyrins, membranes and mitochondria are such structures. Photosensitized damage to mitochondria and membranes has been observed by several techniques. The incubation time with sensitizers is critical since the rates of accumulation of sensitizer are different at different cellular locations. Several other aspects of the action of photosensitizers are reviewed: Have sublethal doses any effect on proliferation and DNA? How can photodynamic treatment act selectively on tumour tissue? Should photodynamic cancer treatment be combined with other therapies? Does photodynamic therapy work under low oxygen concentrations?

Key words

Photosensitization Porphyrins Cancer therapy Membrane damage 

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Copyright information

© Baillière Tindall 1986

Authors and Affiliations

  • Johan Moan
    • 1
  1. 1.Norsk Hydro's Institute for Cancer ResearchThe Norwegian Radium HospitalOslo 3Norway

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